Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors
There is strong evidence that class I MHC-restricted parasite-specific CD8+ CTL protect cattle against the protozoan parasite Theileria parva. As part of an effort to develop a subunit vaccine for the induction of these responses, we have investigated the factors involved in the generation of T. par...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
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1997
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| Online Access: | https://hdl.handle.net/10568/28924 |
| _version_ | 1855531968572162048 |
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| author | Taracha, E.L.N. Awino, Elias McKeever, Declan J. |
| author_browse | Awino, Elias McKeever, Declan J. Taracha, E.L.N. |
| author_facet | Taracha, E.L.N. Awino, Elias McKeever, Declan J. |
| author_sort | Taracha, E.L.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | There is strong evidence that class I MHC-restricted parasite-specific CD8+ CTL protect cattle against the protozoan parasite Theileria parva. As part of an effort to develop a subunit vaccine for the induction of these responses, we have investigated the factors involved in the generation of T. parva-specific CTL in cattle. Purified populations of bovine immune and naive CD8+ T cells were cocultured with autologous T. parva-infected lymphoblasts (TpL) in the presence or absence of immune CD4+ T cells or cytokine preparations. Neither population developed CTL activity when cultured with TpL alone, whereas incorporation of immune CD4+ T cells in the cultures supported the generation of parasite-specific CTL from both immune and naive CD8+ precursors. The helper function of parasite-specific CD4+ T cells for immune, but not naive, CTL precursors could be replaced by CD4+ T cells responding to an unrelated Ag or by the addition of T cell growth factors or recombinant bovine IL-2. In experiments with two-chamber culture plates, in which cocultures of CD4+ and CD8+ T cells with TpL were separated by a semipermeable membrane, CTL activity was observed to develop only in immune precursor populations. Hence, although bovine T. parva-specific CD8+ memory T cells need no helper signals other than IL-2 for activation, their naive counterparts require close contact with responding parasite-specific CD4+T cells. This may reflect essential receptor-ligand interactions, or alternatively, a requirement for more stringent microenvironmental cytokine conditions. |
| format | Journal Article |
| id | CGSpace28924 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| record_format | dspace |
| spelling | CGSpace289242022-01-29T16:19:20Z Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors Taracha, E.L.N. Awino, Elias McKeever, Declan J. theileria parva antigens immunity There is strong evidence that class I MHC-restricted parasite-specific CD8+ CTL protect cattle against the protozoan parasite Theileria parva. As part of an effort to develop a subunit vaccine for the induction of these responses, we have investigated the factors involved in the generation of T. parva-specific CTL in cattle. Purified populations of bovine immune and naive CD8+ T cells were cocultured with autologous T. parva-infected lymphoblasts (TpL) in the presence or absence of immune CD4+ T cells or cytokine preparations. Neither population developed CTL activity when cultured with TpL alone, whereas incorporation of immune CD4+ T cells in the cultures supported the generation of parasite-specific CTL from both immune and naive CD8+ precursors. The helper function of parasite-specific CD4+ T cells for immune, but not naive, CTL precursors could be replaced by CD4+ T cells responding to an unrelated Ag or by the addition of T cell growth factors or recombinant bovine IL-2. In experiments with two-chamber culture plates, in which cocultures of CD4+ and CD8+ T cells with TpL were separated by a semipermeable membrane, CTL activity was observed to develop only in immune precursor populations. Hence, although bovine T. parva-specific CD8+ memory T cells need no helper signals other than IL-2 for activation, their naive counterparts require close contact with responding parasite-specific CD4+T cells. This may reflect essential receptor-ligand interactions, or alternatively, a requirement for more stringent microenvironmental cytokine conditions. 1997 2013-05-06T07:01:45Z 2013-05-06T07:01:45Z Journal Article https://hdl.handle.net/10568/28924 en Limited Access Journal of Immunology;159: 4539-4545 |
| spellingShingle | theileria parva antigens immunity Taracha, E.L.N. Awino, Elias McKeever, Declan J. Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title | Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title_full | Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title_fullStr | Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title_full_unstemmed | Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title_short | Distinct CD4 + T cell helper requirements in Theileria parva-immune and -naive bovine CTL precursors |
| title_sort | distinct cd4 t cell helper requirements in theileria parva immune and naive bovine ctl precursors |
| topic | theileria parva antigens immunity |
| url | https://hdl.handle.net/10568/28924 |
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