An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells
The studies reported here describe methodology permiting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay. We...
| Autores principales: | , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1995
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/28908 |
| _version_ | 1855541323876007936 |
|---|---|
| author | Toye, Philip G. Wijngaard, P.L.J. MacHugh, Niall D. Clevers, H. |
| author_browse | Clevers, H. MacHugh, Niall D. Toye, Philip G. Wijngaard, P.L.J. |
| author_facet | Toye, Philip G. Wijngaard, P.L.J. MacHugh, Niall D. Clevers, H. |
| author_sort | Toye, Philip G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The studies reported here describe methodology permiting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay. We then showed that alloreactive CTL can detect cells expressing the bovine class I MHC molecule in a population of cells transfected with the plasmid containing the corresponding gene plus 100-fold as many plasmids containing an irrelevant gene. In addition, the transiently transfected COS cells can specificially restimulate CTL as detected by a standard microcytotoxicity assay using the target cell line. Overall, the results suggest that COS cells could be employed for the direct screening of an antigen or antigen gene library by immune CTL. |
| format | Journal Article |
| id | CGSpace28908 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace289082023-12-08T19:36:04Z An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells Toye, Philip G. Wijngaard, P.L.J. MacHugh, Niall D. Clevers, H. lymphocytes antigenes cells monoclonal antibodies The studies reported here describe methodology permiting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay. We then showed that alloreactive CTL can detect cells expressing the bovine class I MHC molecule in a population of cells transfected with the plasmid containing the corresponding gene plus 100-fold as many plasmids containing an irrelevant gene. In addition, the transiently transfected COS cells can specificially restimulate CTL as detected by a standard microcytotoxicity assay using the target cell line. Overall, the results suggest that COS cells could be employed for the direct screening of an antigen or antigen gene library by immune CTL. 1995-11 2013-05-06T07:01:43Z 2013-05-06T07:01:43Z Journal Article https://hdl.handle.net/10568/28908 en Limited Access Elsevier Journal of Immunological Methods;187(1): 95-101 |
| spellingShingle | lymphocytes antigenes cells monoclonal antibodies Toye, Philip G. Wijngaard, P.L.J. MacHugh, Niall D. Clevers, H. An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title | An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title_full | An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title_fullStr | An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title_full_unstemmed | An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title_short | An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells |
| title_sort | assay for the identification of antigens recognized by cytotoxic t cells based on the transient transfection of cos cells |
| topic | lymphocytes antigenes cells monoclonal antibodies |
| url | https://hdl.handle.net/10568/28908 |
| work_keys_str_mv | AT toyephilipg anassayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT wijngaardplj anassayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT machughnialld anassayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT cleversh anassayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT toyephilipg assayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT wijngaardplj assayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT machughnialld assayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells AT cleversh assayfortheidentificationofantigensrecognizedbycytotoxictcellsbasedonthetransienttransfectionofcoscells |