Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites
A hybridoma cell line has been previously produced which secretes monoclonal antibodies that neutralize sporozoites of T. parva. Cells from this line were injected i/p into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily i/p injections of a mixt...
| Main Authors: | , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1985
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28904 |
| _version_ | 1855532910717698048 |
|---|---|
| author | Dobbelaere, D.A.E. Spooner, P.R. |
| author_browse | Dobbelaere, D.A.E. Spooner, P.R. |
| author_facet | Dobbelaere, D.A.E. Spooner, P.R. |
| author_sort | Dobbelaere, D.A.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A hybridoma cell line has been previously produced which secretes monoclonal antibodies that neutralize sporozoites of T. parva. Cells from this line were injected i/p into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily i/p injections of a mixture of tritiated amino acids to radiolabel the monoclonal antibody being produced in ascites fluid. The specific activity of the antibody obtained was 100 mCi/mmol. The labelled antibody was used to detect, by autoradiography, a surface coat antigen of T. parva sporozoites in cryostat sections of Theileria-infected tick salivary glands. The method allows the preparation of large quantities of biosynthetically radiolabelled immunological probes for the detection of immunoreactive sites in biological specimens. |
| format | Journal Article |
| id | CGSpace28904 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1985 |
| publishDateRange | 1985 |
| publishDateSort | 1985 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace289042023-12-08T19:36:04Z Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites Dobbelaere, D.A.E. Spooner, P.R. theileria parva monoclonal antibodies radioisotopes antigens cells chemistry immunology parasites protozoal infections A hybridoma cell line has been previously produced which secretes monoclonal antibodies that neutralize sporozoites of T. parva. Cells from this line were injected i/p into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily i/p injections of a mixture of tritiated amino acids to radiolabel the monoclonal antibody being produced in ascites fluid. The specific activity of the antibody obtained was 100 mCi/mmol. The labelled antibody was used to detect, by autoradiography, a surface coat antigen of T. parva sporozoites in cryostat sections of Theileria-infected tick salivary glands. The method allows the preparation of large quantities of biosynthetically radiolabelled immunological probes for the detection of immunoreactive sites in biological specimens. 1985-01 2013-05-06T07:01:42Z 2013-05-06T07:01:42Z Journal Article https://hdl.handle.net/10568/28904 en Limited Access Elsevier Journal of Immunological Methods;82(2): 209-214 |
| spellingShingle | theileria parva monoclonal antibodies radioisotopes antigens cells chemistry immunology parasites protozoal infections Dobbelaere, D.A.E. Spooner, P.R. Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title | Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title_full | Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title_fullStr | Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title_full_unstemmed | Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title_short | Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites |
| title_sort | production in ascites fluid of biosynthetically labelled monoclonal antibody to theileria parva sporozoites |
| topic | theileria parva monoclonal antibodies radioisotopes antigens cells chemistry immunology parasites protozoal infections |
| url | https://hdl.handle.net/10568/28904 |
| work_keys_str_mv | AT dobbelaeredae productioninascitesfluidofbiosyntheticallylabelledmonoclonalantibodytotheileriaparvasporozoites AT spoonerpr productioninascitesfluidofbiosyntheticallylabelledmonoclonalantibodytotheileriaparvasporozoites |