DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships
ABSTRACT. Using the polymerase chain reaction and arbitrarily selected oligonucleotide primers of 10 or 11 bases, we have amplified DNA sequences from Trypanosoma vivax parasites isolated from South America and Africa. On the basis of polymorphisms in the DNA fingerprints generated by three of the p...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1993
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28897 |
| _version_ | 1855532305112629248 |
|---|---|
| author | Dirie, M.F. Murphy, N.B. Gardiner, Peter R. |
| author_browse | Dirie, M.F. Gardiner, Peter R. Murphy, N.B. |
| author_facet | Dirie, M.F. Murphy, N.B. Gardiner, Peter R. |
| author_sort | Dirie, M.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | ABSTRACT. Using the polymerase chain reaction and arbitrarily selected oligonucleotide primers of 10 or 11 bases, we have amplified DNA sequences from Trypanosoma vivax parasites isolated from South America and Africa. On the basis of polymorphisms in the DNA fingerprints generated by three of the primers, the parasites could be separated into two major groups, one comprising T. vivax isolates from Kenya and the second including all the other T. vivax parasites (from Colombia, The Gambia, Nigeria and Uganda). One of these three primers (ILo 525) also gave isolate‐specific DNA fingerprints for the parasites for the parasites tested, which will allow the use of this technique both in the species identification and discrimination of T. vivax parasites. |
| format | Journal Article |
| id | CGSpace28897 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace288972024-05-01T08:18:29Z DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships Dirie, M.F. Murphy, N.B. Gardiner, Peter R. dna trypanosomiasis trypanosoma vivax animal diseases ABSTRACT. Using the polymerase chain reaction and arbitrarily selected oligonucleotide primers of 10 or 11 bases, we have amplified DNA sequences from Trypanosoma vivax parasites isolated from South America and Africa. On the basis of polymorphisms in the DNA fingerprints generated by three of the primers, the parasites could be separated into two major groups, one comprising T. vivax isolates from Kenya and the second including all the other T. vivax parasites (from Colombia, The Gambia, Nigeria and Uganda). One of these three primers (ILo 525) also gave isolate‐specific DNA fingerprints for the parasites for the parasites tested, which will allow the use of this technique both in the species identification and discrimination of T. vivax parasites. 1993-03 2013-05-06T07:01:41Z 2013-05-06T07:01:41Z Journal Article https://hdl.handle.net/10568/28897 en Limited Access Wiley Journal of Eukaryotic Microbiology;40: 132-134 |
| spellingShingle | dna trypanosomiasis trypanosoma vivax animal diseases Dirie, M.F. Murphy, N.B. Gardiner, Peter R. DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title | DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title_full | DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title_fullStr | DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title_full_unstemmed | DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title_short | DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| title_sort | dna fingerprinting of trypanosoma vivax isolates rapidly identifies intraspecific relationships |
| topic | dna trypanosomiasis trypanosoma vivax animal diseases |
| url | https://hdl.handle.net/10568/28897 |
| work_keys_str_mv | AT diriemf dnafingerprintingoftrypanosomavivaxisolatesrapidlyidentifiesintraspecificrelationships AT murphynb dnafingerprintingoftrypanosomavivaxisolatesrapidlyidentifiesintraspecificrelationships AT gardinerpeterr dnafingerprintingoftrypanosomavivaxisolatesrapidlyidentifiesintraspecificrelationships |