Population genetic structure and cladistic analysis of Trypanosoma brucei isolates
Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then perf...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2003
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28769 |
| _version_ | 1855531157014183936 |
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| author | Agbo, E.C. Clausen, Peter-Henning Büscher, Philippe Majiwa, Phelix A.O. Claassen, E. Marinus, F.W.P. |
| author_browse | Agbo, E.C. Büscher, Philippe Claassen, E. Clausen, Peter-Henning Majiwa, Phelix A.O. Marinus, F.W.P. |
| author_facet | Agbo, E.C. Clausen, Peter-Henning Büscher, Philippe Majiwa, Phelix A.O. Claassen, E. Marinus, F.W.P. |
| author_sort | Agbo, E.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei. |
| format | Journal Article |
| id | CGSpace28769 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace287692024-04-25T06:01:17Z Population genetic structure and cladistic analysis of Trypanosoma brucei isolates Agbo, E.C. Clausen, Peter-Henning Büscher, Philippe Majiwa, Phelix A.O. Claassen, E. Marinus, F.W.P. trypanosoma brucei population genetics dna genetic markers animals mankind trypanosoma gambiens trypanosomiasis epidemiology Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei. 2003-09 2013-05-06T07:01:22Z 2013-05-06T07:01:22Z Journal Article https://hdl.handle.net/10568/28769 en Limited Access Elsevier Infection, Genetics and Evolution;3(3): 165-174 |
| spellingShingle | trypanosoma brucei population genetics dna genetic markers animals mankind trypanosoma gambiens trypanosomiasis epidemiology Agbo, E.C. Clausen, Peter-Henning Büscher, Philippe Majiwa, Phelix A.O. Claassen, E. Marinus, F.W.P. Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title | Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title_full | Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title_fullStr | Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title_full_unstemmed | Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title_short | Population genetic structure and cladistic analysis of Trypanosoma brucei isolates |
| title_sort | population genetic structure and cladistic analysis of trypanosoma brucei isolates |
| topic | trypanosoma brucei population genetics dna genetic markers animals mankind trypanosoma gambiens trypanosomiasis epidemiology |
| url | https://hdl.handle.net/10568/28769 |
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