Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium
Cattle were successfully immunized against heartwater with a lysate of Cowdria ruminantium formulated in Freund's adjuvant. Vaccinated animals proved fully resistant to virulent challenge 3 and 10 months after vaccination. For the first time a helper T lymphocyte response to Cowdria antigens was obs...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1997
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28765 |
| _version_ | 1855533951634898944 |
|---|---|
| author | Totte, P. McKeever, Declan J. Martínez, D. Bensaid, A. |
| author_browse | Bensaid, A. Martínez, D. McKeever, Declan J. Totte, P. |
| author_facet | Totte, P. McKeever, Declan J. Martínez, D. Bensaid, A. |
| author_sort | Totte, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cattle were successfully immunized against heartwater with a lysate of Cowdria ruminantium formulated in Freund's adjuvant. Vaccinated animals proved fully resistant to virulent challenge 3 and 10 months after vaccination. For the first time a helper T lymphocyte response to Cowdria antigens was observed and characterized. Cowdria-specific T-cell lines generated from vaccinated animals by in vitro restimulation with Cowdria lysates are 95 to 100 percent CD4+, are MHC class II restricted, and produce gamma interferon. They proliferate in response to autologous monocytes infected with live Cowdria but not in response to uninfected monocytes. These T-cell lines will facilitate the search for Cowdria antigens that are immunogenic for T cells and will therefore be of relevance in the development of a subunit vaccine against the disease. |
| format | Journal Article |
| id | CGSpace28765 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| record_format | dspace |
| spelling | CGSpace287652022-01-29T16:16:36Z Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium Totte, P. McKeever, Declan J. Martínez, D. Bensaid, A. cowdria ruminantium cattle immunization vaccination cowdriosis Cattle were successfully immunized against heartwater with a lysate of Cowdria ruminantium formulated in Freund's adjuvant. Vaccinated animals proved fully resistant to virulent challenge 3 and 10 months after vaccination. For the first time a helper T lymphocyte response to Cowdria antigens was observed and characterized. Cowdria-specific T-cell lines generated from vaccinated animals by in vitro restimulation with Cowdria lysates are 95 to 100 percent CD4+, are MHC class II restricted, and produce gamma interferon. They proliferate in response to autologous monocytes infected with live Cowdria but not in response to uninfected monocytes. These T-cell lines will facilitate the search for Cowdria antigens that are immunogenic for T cells and will therefore be of relevance in the development of a subunit vaccine against the disease. 1997 2013-05-06T07:01:22Z 2013-05-06T07:01:22Z Journal Article https://hdl.handle.net/10568/28765 en Limited Access Infection and Immunity;65(1): 236-241 |
| spellingShingle | cowdria ruminantium cattle immunization vaccination cowdriosis Totte, P. McKeever, Declan J. Martínez, D. Bensaid, A. Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title | Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title_full | Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title_fullStr | Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title_full_unstemmed | Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title_short | Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium |
| title_sort | analysis of t cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of cowdria ruminantium |
| topic | cowdria ruminantium cattle immunization vaccination cowdriosis |
| url | https://hdl.handle.net/10568/28765 |
| work_keys_str_mv | AT tottep analysisoftcellresponsesincattleimmunizedagainstheartwaterbyvaccinationwithkilledelementarybodiesofcowdriaruminantium AT mckeeverdeclanj analysisoftcellresponsesincattleimmunizedagainstheartwaterbyvaccinationwithkilledelementarybodiesofcowdriaruminantium AT martinezd analysisoftcellresponsesincattleimmunizedagainstheartwaterbyvaccinationwithkilledelementarybodiesofcowdriaruminantium AT bensaida analysisoftcellresponsesincattleimmunizedagainstheartwaterbyvaccinationwithkilledelementarybodiesofcowdriaruminantium |