Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes
Peripheral blood mononuclear cells (PBMC) from immune cattle proliferate in the presence of autologous Cowdria ruminantium-infected endothelial cells and monocytes. Endothelial cells required treatment with T-cell growth factors to induce class II major histocompatibility complex expression prior to...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Society for Microbiology
1998
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28756 |
| _version_ | 1855524892518121472 |
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| author | Mwangi, David Miano Mahan, S.M. Nyanjui, J.K. Taracha, E.L.N. McKeever, Declan J. |
| author_browse | Mahan, S.M. McKeever, Declan J. Mwangi, David Miano Nyanjui, J.K. Taracha, E.L.N. |
| author_facet | Mwangi, David Miano Mahan, S.M. Nyanjui, J.K. Taracha, E.L.N. McKeever, Declan J. |
| author_sort | Mwangi, David Miano |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Peripheral blood mononuclear cells (PBMC) from immune cattle proliferate in the presence of autologous Cowdria ruminantium-infected endothelial cells and monocytes. Endothelial cells required treatment with T-cell growth factors to induce class II major histocompatibility complex expression prior to infection and use as stimulators. Proliferative responses to both infected autologous endothelial cells and monocytes were characterized by expansion of a mixture of CD4+, CD8+, and gamma delta T cells. However, gamma delta T cells dominated following several restimulations. Reverse transcription-PCR analysis of cytokine expression by C. ruminantium-specific T-cell lines and immune PBMC revealed weak interleukin-2 (IL-2), IL-4, and gamma interferon (IFN-y) transcripts at 3 to 24 h after stimulation. Strong expression of IFN-y, tumor necrosis factor alpha (TNF-a)m TNF-beta, and IL-2 receptor a-chain mRNA was detected in T-cell lines 48 h after antigen stimulation. Super-natants from these T-cell cultures contained IFN-y protein. Our findings suggest that in immune cattle a C. ruminantium-specific T-cell response is induced and that infected endothelial cells and monocytes may present C. ruminantium antigens to specific T lymphocytes in vivo during infection and thereby play a role in induction of protective immune responses to the pathogen. |
| format | Journal Article |
| id | CGSpace28756 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace287562024-03-06T10:16:43Z Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes Mwangi, David Miano Mahan, S.M. Nyanjui, J.K. Taracha, E.L.N. McKeever, Declan J. cowdria ruminantium cattle immunization lymphocytes monocytes Peripheral blood mononuclear cells (PBMC) from immune cattle proliferate in the presence of autologous Cowdria ruminantium-infected endothelial cells and monocytes. Endothelial cells required treatment with T-cell growth factors to induce class II major histocompatibility complex expression prior to infection and use as stimulators. Proliferative responses to both infected autologous endothelial cells and monocytes were characterized by expansion of a mixture of CD4+, CD8+, and gamma delta T cells. However, gamma delta T cells dominated following several restimulations. Reverse transcription-PCR analysis of cytokine expression by C. ruminantium-specific T-cell lines and immune PBMC revealed weak interleukin-2 (IL-2), IL-4, and gamma interferon (IFN-y) transcripts at 3 to 24 h after stimulation. Strong expression of IFN-y, tumor necrosis factor alpha (TNF-a)m TNF-beta, and IL-2 receptor a-chain mRNA was detected in T-cell lines 48 h after antigen stimulation. Super-natants from these T-cell cultures contained IFN-y protein. Our findings suggest that in immune cattle a C. ruminantium-specific T-cell response is induced and that infected endothelial cells and monocytes may present C. ruminantium antigens to specific T lymphocytes in vivo during infection and thereby play a role in induction of protective immune responses to the pathogen. 1998-05 2013-05-06T07:01:21Z 2013-05-06T07:01:21Z Journal Article https://hdl.handle.net/10568/28756 en Open Access American Society for Microbiology Infection and Immunity;66(5): 1855-1860 |
| spellingShingle | cowdria ruminantium cattle immunization lymphocytes monocytes Mwangi, David Miano Mahan, S.M. Nyanjui, J.K. Taracha, E.L.N. McKeever, Declan J. Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title | Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title_full | Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title_fullStr | Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title_full_unstemmed | Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title_short | Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes |
| title_sort | immunization of cattle by infection with cowdria ruminantium elicits t lymphocytes that recognize autologous infected endothelial cells and monocytes |
| topic | cowdria ruminantium cattle immunization lymphocytes monocytes |
| url | https://hdl.handle.net/10568/28756 |
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