Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle
Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with trypan...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1995
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28726 |
| _version_ | 1855540915708362752 |
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| author | Lutje, V. Taylor, K.A. Boulange, A.F. Authié, Edith |
| author_browse | Authié, Edith Boulange, A.F. Lutje, V. Taylor, K.A. |
| author_facet | Lutje, V. Taylor, K.A. Boulange, A.F. Authié, Edith |
| author_sort | Lutje, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with trypanocidal drugs following each infection and had remained aparasitaemic for 3 years prior to this study. The antigens used were whole trypanosome lysate, variable surface glycoprotein, a 33thin000 MW cysteine protease (congopain) and a 70thin000 MW heat-shock protein. As parameters of T-cell-mediated immunity, T-cell proliferation and IFN-gamma production were monitored. Lymph node cells, spleen cells and peripheral blood mononuclear cells all proliferation to a mitogenic stimulus (concanavalin A) but only lymph node cells responded to trypanosome antigens. Similarly, IFN-gamma was produced by both lymph node and spleen cells stimulated with concanavalin A but only by lymph node cells stimulated with variable surface glycoprotein and whole trypanosome lysate. T. congolense-specific antibodies were detected in sera and in supernatants of cultured lymph node and spleen cells after in vitro stimulation with lipopolysaccharide and recombinant bovine interleukin-2. It is concluded that memory T- and B-cell responses are detectable in various lymphoid organs in cattle 3 years after infection with T. congolense. |
| format | Journal Article |
| id | CGSpace28726 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace287262024-03-06T10:16:43Z Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle Lutje, V. Taylor, K.A. Boulange, A.F. Authié, Edith cytokines livestock trypanosoma congolense n'dama cattle immune response immunization t lymphocytes b lymphocytes interferon interleukin 2 drug therapy chemotherapy disease control animal diseases Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with trypanocidal drugs following each infection and had remained aparasitaemic for 3 years prior to this study. The antigens used were whole trypanosome lysate, variable surface glycoprotein, a 33thin000 MW cysteine protease (congopain) and a 70thin000 MW heat-shock protein. As parameters of T-cell-mediated immunity, T-cell proliferation and IFN-gamma production were monitored. Lymph node cells, spleen cells and peripheral blood mononuclear cells all proliferation to a mitogenic stimulus (concanavalin A) but only lymph node cells responded to trypanosome antigens. Similarly, IFN-gamma was produced by both lymph node and spleen cells stimulated with concanavalin A but only by lymph node cells stimulated with variable surface glycoprotein and whole trypanosome lysate. T. congolense-specific antibodies were detected in sera and in supernatants of cultured lymph node and spleen cells after in vitro stimulation with lipopolysaccharide and recombinant bovine interleukin-2. It is concluded that memory T- and B-cell responses are detectable in various lymphoid organs in cattle 3 years after infection with T. congolense. 1995-11 2013-05-06T07:01:16Z 2013-05-06T07:01:16Z Journal Article https://hdl.handle.net/10568/28726 en Limited Access Elsevier Immunology Letters;48(1): 29-34 |
| spellingShingle | cytokines livestock trypanosoma congolense n'dama cattle immune response immunization t lymphocytes b lymphocytes interferon interleukin 2 drug therapy chemotherapy disease control animal diseases Lutje, V. Taylor, K.A. Boulange, A.F. Authié, Edith Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title | Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title_full | Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title_fullStr | Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title_full_unstemmed | Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title_short | Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle |
| title_sort | trypanosoma congolense tissue distribution of long term t and b cell responses in cattle |
| topic | cytokines livestock trypanosoma congolense n'dama cattle immune response immunization t lymphocytes b lymphocytes interferon interleukin 2 drug therapy chemotherapy disease control animal diseases |
| url | https://hdl.handle.net/10568/28726 |
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