Application of molecular tools in support of deployment of Theileria parva live vaccines
The primary goals of applying molecular tools, developed at ILRI in conjunction with the use of infection and treatment immunisation are quality control of vaccines; monitoring of possible vaccine breakthrough and breakdown; and assessment of the impact of introducing new parasite types on long-term...
| Autores principales: | , , , , , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
| Publicado: |
International Livestock Research Institute
1999
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/2865 |
| Sumario: | The primary goals of applying molecular tools, developed at ILRI in conjunction with the use of infection and treatment immunisation are quality control of vaccines; monitoring of possible vaccine breakthrough and breakdown; and assessment of the impact of introducing new parasite types on long-term efficacy of the vaccine. There are two main categories of tools available for use; serological such as schizont - specific monoclonal antibodies and DNA-based. This paper outlines serological techniques; and DNA-based techniques including southern blotting, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP). It discusses quality control and markers for vaccine stocks; investigating possible vaccine breakdown and breakthrough; molecular epidemiology of ECF which deals with role of other theileria species, genetic variation between T. parva isolates and population genetics. |
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