Cloning of the bovine interleukin-3-encoding cDNA
Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared fro...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
1995
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28649 |
| _version_ | 1855513533095084032 |
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| author | Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. |
| author_browse | Logan-Henfrey, L.L. McInnes, C. Mertens, B. Mwangi, S.M. |
| author_facet | Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. |
| author_sort | Mwangi, S.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7.55.8 and 51.9 percent nt identity, respectively, in the coding region, and 85.4.35 and 27.7 percent aa identity respectively. |
| format | Journal Article |
| id | CGSpace28649 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace286492024-11-18T08:28:57Z Cloning of the bovine interleukin-3-encoding cDNA Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. cattle dna cytology blood cells molecular cloning nucleotide sequence genetics Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7.55.8 and 51.9 percent nt identity, respectively, in the coding region, and 85.4.35 and 27.7 percent aa identity respectively. 1995-09 2013-05-06T07:01:05Z 2013-05-06T07:01:05Z Journal Article https://hdl.handle.net/10568/28649 en Limited Access Elsevier Mwangi, S. M., Logan-Henfrey, L., McInnes, C., & Mertens, B. (1995). Cloning of the bovine interleukin-3-encoding cDNA. Gene 162(2): 309–312. Elsevier BV. https://doi.org/10.1016/0378-1119(95)00359-e |
| spellingShingle | cattle dna cytology blood cells molecular cloning nucleotide sequence genetics Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. Cloning of the bovine interleukin-3-encoding cDNA |
| title | Cloning of the bovine interleukin-3-encoding cDNA |
| title_full | Cloning of the bovine interleukin-3-encoding cDNA |
| title_fullStr | Cloning of the bovine interleukin-3-encoding cDNA |
| title_full_unstemmed | Cloning of the bovine interleukin-3-encoding cDNA |
| title_short | Cloning of the bovine interleukin-3-encoding cDNA |
| title_sort | cloning of the bovine interleukin 3 encoding cdna |
| topic | cattle dna cytology blood cells molecular cloning nucleotide sequence genetics |
| url | https://hdl.handle.net/10568/28649 |
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