Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection

A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation...

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Main Authors: Lutje, V., Taylor, K.A., Kennedy, D., Authié, Edith, Boulange, A.F., Gettinby, George
Format: Journal Article
Language:Inglés
Published: Elsevier 1996
Subjects:
Online Access:https://hdl.handle.net/10568/28611
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author Lutje, V.
Taylor, K.A.
Kennedy, D.
Authié, Edith
Boulange, A.F.
Gettinby, George
author_browse Authié, Edith
Boulange, A.F.
Gettinby, George
Kennedy, D.
Lutje, V.
Taylor, K.A.
author_facet Lutje, V.
Taylor, K.A.
Kennedy, D.
Authié, Edith
Boulange, A.F.
Gettinby, George
author_sort Lutje, V.
collection Repository of Agricultural Research Outputs (CGSpace)
description A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-y synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in the in vitro proliferation of LNC to VSG, to hsp 70/BiP, or to Con A were detected between the breeds. In contrast, IFN-y production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cell and an increase in the percentage of B-cells, CD8+T-cells, and yo T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis to lymph node cells.
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spelling CGSpace286112024-05-01T08:17:29Z Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection Lutje, V. Taylor, K.A. Kennedy, D. Authié, Edith Boulange, A.F. Gettinby, George trypanosomiasis bovidae trypanosoma congolense disease resistance lymphatic system infection cattle A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-y synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in the in vitro proliferation of LNC to VSG, to hsp 70/BiP, or to Con A were detected between the breeds. In contrast, IFN-y production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cell and an increase in the percentage of B-cells, CD8+T-cells, and yo T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis to lymph node cells. 1996-12 2013-05-06T07:00:59Z 2013-05-06T07:00:59Z Journal Article https://hdl.handle.net/10568/28611 en Limited Access Elsevier Experimental Parasitology;84: 320-329
spellingShingle trypanosomiasis
bovidae
trypanosoma congolense
disease resistance
lymphatic system
infection
cattle
Lutje, V.
Taylor, K.A.
Kennedy, D.
Authié, Edith
Boulange, A.F.
Gettinby, George
Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title_full Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title_fullStr Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title_full_unstemmed Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title_short Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
title_sort trypanosoma congolense a comparison of t cell mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection
topic trypanosomiasis
bovidae
trypanosoma congolense
disease resistance
lymphatic system
infection
cattle
url https://hdl.handle.net/10568/28611
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