Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences
Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species variable region, located betwee...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1995
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28604 |
| _version_ | 1855534591362727936 |
|---|---|
| author | Bishop, Richard P. Allsopp, B.A. Spooner, P.R. Sohanpal, B.K. Morzaria, S.P. Gobright, E.I. |
| author_browse | Allsopp, B.A. Bishop, Richard P. Gobright, E.I. Morzaria, S.P. Sohanpal, B.K. Spooner, P.R. |
| author_facet | Bishop, Richard P. Allsopp, B.A. Spooner, P.R. Sohanpal, B.K. Morzaria, S.P. Gobright, E.I. |
| author_sort | Bishop, Richard P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species variable region, located between the first and second conserved secondary structure domains of LSU rRNA, was identified. The sequence of the major species-variable region was determined for T. annulata. T, buffeli, and an unclassified Theileria species isolated from buffalo. Species-specific oligonucleotides complementary to LSU rRNA sequences were designed for five species of Theileria. The oligonucleotide probes discriminated between Theileria species when hybridized to amplied parasite LSU rRNA genes or directly to parasite rRNA. Three species-specific oligonuceotides derived from nonoverlapping sequences were developed for each of T. parva and T. taurotragi. A simplified procedure was developed in which T. parva- and T. taurotragi-specific oligonucleotides were used to discriminate the two species under nonstringent hybridization and washing conditions. Use of the nonstringent conditions also increased the sensitivity of target detection. Comparison of Theileria species-specific oligonucleotides derived from large subunit and small subunit rRNA genes demonstrated that, for closely related species, probes derived from LSU rRNA sequences had improved specificity. |
| format | Journal Article |
| id | CGSpace28604 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace286042024-05-01T08:18:50Z Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences Bishop, Richard P. Allsopp, B.A. Spooner, P.R. Sohanpal, B.K. Morzaria, S.P. Gobright, E.I. theileriosis east coast fever genes oligonucleotide animal diseases Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species variable region, located between the first and second conserved secondary structure domains of LSU rRNA, was identified. The sequence of the major species-variable region was determined for T. annulata. T, buffeli, and an unclassified Theileria species isolated from buffalo. Species-specific oligonucleotides complementary to LSU rRNA sequences were designed for five species of Theileria. The oligonucleotide probes discriminated between Theileria species when hybridized to amplied parasite LSU rRNA genes or directly to parasite rRNA. Three species-specific oligonuceotides derived from nonoverlapping sequences were developed for each of T. parva and T. taurotragi. A simplified procedure was developed in which T. parva- and T. taurotragi-specific oligonucleotides were used to discriminate the two species under nonstringent hybridization and washing conditions. Use of the nonstringent conditions also increased the sensitivity of target detection. Comparison of Theileria species-specific oligonucleotides derived from large subunit and small subunit rRNA genes demonstrated that, for closely related species, probes derived from LSU rRNA sequences had improved specificity. 1995-02 2013-05-06T07:00:58Z 2013-05-06T07:00:58Z Journal Article https://hdl.handle.net/10568/28604 en Limited Access Elsevier Experimental Parasitology;80: 107-115 |
| spellingShingle | theileriosis east coast fever genes oligonucleotide animal diseases Bishop, Richard P. Allsopp, B.A. Spooner, P.R. Sohanpal, B.K. Morzaria, S.P. Gobright, E.I. Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title | Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title_full | Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title_fullStr | Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title_full_unstemmed | Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title_short | Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences |
| title_sort | theileria improved species discrimination using oligonucleotide derived from large subunit ribosomal rna sequences |
| topic | theileriosis east coast fever genes oligonucleotide animal diseases |
| url | https://hdl.handle.net/10568/28604 |
| work_keys_str_mv | AT bishoprichardp theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences AT allsoppba theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences AT spoonerpr theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences AT sohanpalbk theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences AT morzariasp theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences AT gobrightei theileriaimprovedspeciesdiscriminationusingoligonucleotidederivedfromlargesubunitribosomalrnasequences |