Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes
High-resolution mapping of quantitative trait loci (QTLs) is an essential step towards positional cloning and identification of the corresponding genes. Most QTL detection and mapping studies in mice have been carried out using F2 intercross and backcross populations. As a consequence of the limited...
| Autor principal: | |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Informa UK Limited
2000
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| Acceso en línea: | https://hdl.handle.net/10568/28577 |
| _version_ | 1855539129705562112 |
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| author | Iraqi, F.A. |
| author_browse | Iraqi, F.A. |
| author_facet | Iraqi, F.A. |
| author_sort | Iraqi, F.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | High-resolution mapping of quantitative trait loci (QTLs) is an essential step towards positional cloning and identification of the corresponding genes. Most QTL detection and mapping studies in mice have been carried out using F2 intercross and backcross populations. As a consequence of the limited number of recombination events in small chromosomal regions, this has generally permitted mapping to only relatively large confidence intervals of 20 to 40 cM. A number of po pulation designs have been proposed to increase recombination level in crosses. This includes advanced intercross lines (AIL) described by Daruasi and Soller (Genomics. 1995; 141: 1199-1207). In this report demonstration of the utility of the AIL approach for fine mapping of QTL, which previously had been mapped with 95 % confidence interval to 20 to 40 cM in a F2 intercross, will be presented. The methodological approaches to go from the fine-mapped QTL to the identification of the actual genes and mutations are discussed. |
| format | Journal Article |
| id | CGSpace28577 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace285772024-05-01T08:19:33Z Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes Iraqi, F.A. genetic maps molecular cloning mice trypanosomiasis disease resistance dna High-resolution mapping of quantitative trait loci (QTLs) is an essential step towards positional cloning and identification of the corresponding genes. Most QTL detection and mapping studies in mice have been carried out using F2 intercross and backcross populations. As a consequence of the limited number of recombination events in small chromosomal regions, this has generally permitted mapping to only relatively large confidence intervals of 20 to 40 cM. A number of po pulation designs have been proposed to increase recombination level in crosses. This includes advanced intercross lines (AIL) described by Daruasi and Soller (Genomics. 1995; 141: 1199-1207). In this report demonstration of the utility of the AIL approach for fine mapping of QTL, which previously had been mapped with 95 % confidence interval to 20 to 40 cM in a F2 intercross, will be presented. The methodological approaches to go from the fine-mapped QTL to the identification of the actual genes and mutations are discussed. 2000-01 2013-05-06T07:00:55Z 2013-05-06T07:00:55Z Journal Article https://hdl.handle.net/10568/28577 en Limited Access Informa UK Limited Experimental Lung Research;26: 641-649 |
| spellingShingle | genetic maps molecular cloning mice trypanosomiasis disease resistance dna Iraqi, F.A. Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title | Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title_full | Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title_fullStr | Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title_full_unstemmed | Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title_short | Fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| title_sort | fine mapping of quantitative trait loci using advanced intercross lines of mice and positional cloning of the corresponding genes |
| topic | genetic maps molecular cloning mice trypanosomiasis disease resistance dna |
| url | https://hdl.handle.net/10568/28577 |
| work_keys_str_mv | AT iraqifa finemappingofquantitativetraitlociusingadvancedintercrosslinesofmiceandpositionalcloningofthecorrespondinggenes |