Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites
A flow cytometric method using anti-parasite antibodies was developed to measure binding of theileria parva sporozoites to the target bovine lymphocyte membrane. Parasite-host cell interactions could be inhibited by monoclonal antibodies to bovine MHC class I and partially by one of two antibodies t...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1998
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28537 |
| _version_ | 1855536641725169664 |
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| author | Syfrig, J. Wells, C. Daubenberger, C.A. Musoke, A.J. Naessens, Jan |
| author_browse | Daubenberger, C.A. Musoke, A.J. Naessens, Jan Syfrig, J. Wells, C. |
| author_facet | Syfrig, J. Wells, C. Daubenberger, C.A. Musoke, A.J. Naessens, Jan |
| author_sort | Syfrig, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A flow cytometric method using anti-parasite antibodies was developed to measure binding of theileria parva sporozoites to the target bovine lymphocyte membrane. Parasite-host cell interactions could be inhibited by monoclonal antibodies to bovine MHC class I and partially by one of two antibodies to BoCD45R. Proteolysis of the lymphocyte surface removed CD45R but not MHC class I determinants, and enhanced sporozoite binding. These observations support the hypothesis that CD45R and CD45R antibodies may non-specifically prevent close approximation between sporozoites and lymphocytes. Interestingly, under normal conditions, sporozoites of T. parva did not attach to lymphocytes from goats, but did so when the cells were treated with the protease, suggesting that receptor(s) for T. parva sporozoites might be present on caprine cells but are not easily accessible. These and other results indicate that proteases may be involved in binding and entry of T. parva sporozoites. Electron microscopy revealed that the process of binding and entry of sporozoites into protease-treated goat lymphocytes was very similar to that of the bovine cells. However, schizonts did not develop and lymphocyte proliferation was not induced, indicating that cell entry by sporozoites and cellular transformation are separate processes. |
| format | Journal Article |
| id | CGSpace28537 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace285372024-05-01T08:19:08Z Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites Syfrig, J. Wells, C. Daubenberger, C.A. Musoke, A.J. Naessens, Jan theileria parva sporozoa disease resistance lymphocytes proteases A flow cytometric method using anti-parasite antibodies was developed to measure binding of theileria parva sporozoites to the target bovine lymphocyte membrane. Parasite-host cell interactions could be inhibited by monoclonal antibodies to bovine MHC class I and partially by one of two antibodies to BoCD45R. Proteolysis of the lymphocyte surface removed CD45R but not MHC class I determinants, and enhanced sporozoite binding. These observations support the hypothesis that CD45R and CD45R antibodies may non-specifically prevent close approximation between sporozoites and lymphocytes. Interestingly, under normal conditions, sporozoites of T. parva did not attach to lymphocytes from goats, but did so when the cells were treated with the protease, suggesting that receptor(s) for T. parva sporozoites might be present on caprine cells but are not easily accessible. These and other results indicate that proteases may be involved in binding and entry of T. parva sporozoites. Electron microscopy revealed that the process of binding and entry of sporozoites into protease-treated goat lymphocytes was very similar to that of the bovine cells. However, schizonts did not develop and lymphocyte proliferation was not induced, indicating that cell entry by sporozoites and cellular transformation are separate processes. 1998-06 2013-05-06T07:00:49Z 2013-05-06T07:00:49Z Journal Article https://hdl.handle.net/10568/28537 en Limited Access Elsevier European Journal of Cell Biology;76: 125-132 |
| spellingShingle | theileria parva sporozoa disease resistance lymphocytes proteases Syfrig, J. Wells, C. Daubenberger, C.A. Musoke, A.J. Naessens, Jan Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title | Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title_full | Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title_fullStr | Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title_full_unstemmed | Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title_short | Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites |
| title_sort | proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by theileria parva sporozoites |
| topic | theileria parva sporozoa disease resistance lymphocytes proteases |
| url | https://hdl.handle.net/10568/28537 |
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