Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs
Procyclic Trypanosoma brucei rhodesiense have a cell death mechanism which can be activated by an external signal, the lectin ConA, in vitro. ConA has been shown to cause profound changes in cellular morphology and induce fragmentation of nuclear DNA in T.b. rhodesiense which are characteristic of a...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1997
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/28412 |
| _version_ | 1855534591172935680 |
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| author | Murphy, N.B. Welburn, S.C. |
| author_browse | Murphy, N.B. Welburn, S.C. |
| author_facet | Murphy, N.B. Welburn, S.C. |
| author_sort | Murphy, N.B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Procyclic Trypanosoma brucei rhodesiense have a cell death mechanism which can be activated by an external signal, the lectin ConA, in vitro. ConA has been shown to cause profound changes in cellular morphology and induce fragmentation of nuclear DNA in T.b. rhodesiense which are characteristic of apoptosis, a form of programmed cell death (PCD) in other eukaryotic cells. RNA analysis of trypanosomes induced to undergo PCD revealed that RNA remains intact up to 48 h into the process, a time when nuclear DNA fragmentation has already started. Using the randomly amplified differentially expressed sequences polymerase chain reaction method, ConA-induced cell death in T.b rhodesiense is shown to be associated with differential expression of mRNAs, including up regulation of mRNAs late in the death process. The results demonstrate that trypanosomes actively participate in their own destruction through a PCD process and confirm that cell death in trypanosomes is associated with de novo gene expression. |
| format | Journal Article |
| id | CGSpace28412 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| record_format | dspace |
| spelling | CGSpace284122022-01-29T16:15:36Z Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs Murphy, N.B. Welburn, S.C. trypanosoma brucei cells rna Procyclic Trypanosoma brucei rhodesiense have a cell death mechanism which can be activated by an external signal, the lectin ConA, in vitro. ConA has been shown to cause profound changes in cellular morphology and induce fragmentation of nuclear DNA in T.b. rhodesiense which are characteristic of apoptosis, a form of programmed cell death (PCD) in other eukaryotic cells. RNA analysis of trypanosomes induced to undergo PCD revealed that RNA remains intact up to 48 h into the process, a time when nuclear DNA fragmentation has already started. Using the randomly amplified differentially expressed sequences polymerase chain reaction method, ConA-induced cell death in T.b rhodesiense is shown to be associated with differential expression of mRNAs, including up regulation of mRNAs late in the death process. The results demonstrate that trypanosomes actively participate in their own destruction through a PCD process and confirm that cell death in trypanosomes is associated with de novo gene expression. 1997 2013-05-06T07:00:33Z 2013-05-06T07:00:33Z Journal Article https://hdl.handle.net/10568/28412 en Limited Access Cell Death and Differentiation;4: 365-370 |
| spellingShingle | trypanosoma brucei cells rna Murphy, N.B. Welburn, S.C. Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title | Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title_full | Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title_fullStr | Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title_full_unstemmed | Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title_short | Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs |
| title_sort | programmed cell death in procyclic trypanosoma brucei rhodesiense is associated with differential expression of mrnas |
| topic | trypanosoma brucei cells rna |
| url | https://hdl.handle.net/10568/28412 |
| work_keys_str_mv | AT murphynb programmedcelldeathinprocyclictrypanosomabruceirhodesienseisassociatedwithdifferentialexpressionofmrnas AT welburnsc programmedcelldeathinprocyclictrypanosomabruceirhodesienseisassociatedwithdifferentialexpressionofmrnas |