B-cell activation in trypanosome-infected Boran cattle
Analysis of serum antibody responses in cattle experimentally infected with Trypanosoma congolense suggested that antibody responses of trypanosusceptible Boran cattle are less efficient in isotype switching and affinity maturation than those of trypanotolerant N'Dama cattle. High levels of polyspec...
| Main Authors: | , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Laboratory for Research on Animal Diseases
1995
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/2825 |
| _version_ | 1855533735066206208 |
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| author | Taylor, K.A. Williams, D.J.L. Lutje, V. |
| author_browse | Lutje, V. Taylor, K.A. Williams, D.J.L. |
| author_facet | Taylor, K.A. Williams, D.J.L. Lutje, V. |
| author_sort | Taylor, K.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Analysis of serum antibody responses in cattle experimentally infected with Trypanosoma congolense suggested that antibody responses of trypanosusceptible Boran cattle are less efficient in isotype switching and affinity maturation than those of trypanotolerant N'Dama cattle. High levels of polyspecific IgM are produced by Boran cattle while N'Dama cattle have higher titres of IgG specific for certain trypanosome antigens. Further characterization of these differences required determination of the frequency and phenotype of antibody secreting cells (ASC), as well as the specificity of the antibody and the isotype secreted in response to infection. A method was therefore developed to maintain bovine B cells in culture and stimulate them to differentiate into ASC. B cells were examined for the expression of the transferrin receptor and the CD5 antigen using fluorescence activated cell sorter (FACS) analysis and the number of ASC was determined using an ELISpot assay. We compared B-cell activation and the frequency of ASC between six Trypanosoma congolense-infected cattle and three uninfected controls. Peripheral blood lymphocytes (PBL) were analysed before and after in vitro culture for three and six days with combinations of lipopolysaccharide (LPS), pokeweed mitogen (PWM) and recombinant bovine interleukin-2 (rboIL 2). Splenic lymphocytes from T. congolense-infected cattle were compared with PBL from the same animals. |
| format | Conference Paper |
| id | CGSpace2825 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | International Laboratory for Research on Animal Diseases |
| publisherStr | International Laboratory for Research on Animal Diseases |
| record_format | dspace |
| spelling | CGSpace28252023-12-21T14:51:44Z B-cell activation in trypanosome-infected Boran cattle Taylor, K.A. Williams, D.J.L. Lutje, V. trypanosomiasis animal diseases lymphocytes antibodies immune response disease control glossinidae ndama cattle trypanosoma congolense Analysis of serum antibody responses in cattle experimentally infected with Trypanosoma congolense suggested that antibody responses of trypanosusceptible Boran cattle are less efficient in isotype switching and affinity maturation than those of trypanotolerant N'Dama cattle. High levels of polyspecific IgM are produced by Boran cattle while N'Dama cattle have higher titres of IgG specific for certain trypanosome antigens. Further characterization of these differences required determination of the frequency and phenotype of antibody secreting cells (ASC), as well as the specificity of the antibody and the isotype secreted in response to infection. A method was therefore developed to maintain bovine B cells in culture and stimulate them to differentiate into ASC. B cells were examined for the expression of the transferrin receptor and the CD5 antigen using fluorescence activated cell sorter (FACS) analysis and the number of ASC was determined using an ELISpot assay. We compared B-cell activation and the frequency of ASC between six Trypanosoma congolense-infected cattle and three uninfected controls. Peripheral blood lymphocytes (PBL) were analysed before and after in vitro culture for three and six days with combinations of lipopolysaccharide (LPS), pokeweed mitogen (PWM) and recombinant bovine interleukin-2 (rboIL 2). Splenic lymphocytes from T. congolense-infected cattle were compared with PBL from the same animals. 1995 2010-12-09T11:11:59Z 2010-12-09T11:11:59Z Conference Paper https://hdl.handle.net/10568/2825 en Open Access International Laboratory for Research on Animal Diseases |
| spellingShingle | trypanosomiasis animal diseases lymphocytes antibodies immune response disease control glossinidae ndama cattle trypanosoma congolense Taylor, K.A. Williams, D.J.L. Lutje, V. B-cell activation in trypanosome-infected Boran cattle |
| title | B-cell activation in trypanosome-infected Boran cattle |
| title_full | B-cell activation in trypanosome-infected Boran cattle |
| title_fullStr | B-cell activation in trypanosome-infected Boran cattle |
| title_full_unstemmed | B-cell activation in trypanosome-infected Boran cattle |
| title_short | B-cell activation in trypanosome-infected Boran cattle |
| title_sort | b cell activation in trypanosome infected boran cattle |
| topic | trypanosomiasis animal diseases lymphocytes antibodies immune response disease control glossinidae ndama cattle trypanosoma congolense |
| url | https://hdl.handle.net/10568/2825 |
| work_keys_str_mv | AT taylorka bcellactivationintrypanosomeinfectedborancattle AT williamsdjl bcellactivationintrypanosomeinfectedborancattle AT lutjev bcellactivationintrypanosomeinfectedborancattle |