A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification
SummaryA polymorphism was detected in the 3′ untranslated region of the bovine gamma‐S‐crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
1993
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/28227 |
| _version_ | 1855524140271796224 |
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| author | Kemp, Stephen J. Maillard, J.C. Teale, A.J. |
| author_browse | Kemp, Stephen J. Maillard, J.C. Teale, A.J. |
| author_facet | Kemp, Stephen J. Maillard, J.C. Teale, A.J. |
| author_sort | Kemp, Stephen J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | SummaryA polymorphism was detected in the 3′ untranslated region of the bovine gamma‐S‐crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele‐specific amplification. The two allele‐specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism. |
| format | Journal Article |
| id | CGSpace28227 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace282272024-05-01T08:17:08Z A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification Kemp, Stephen J. Maillard, J.C. Teale, A.J. cattle genes SummaryA polymorphism was detected in the 3′ untranslated region of the bovine gamma‐S‐crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele‐specific amplification. The two allele‐specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism. 1993 2013-05-06T07:00:11Z 2013-05-06T07:00:11Z Journal Article https://hdl.handle.net/10568/28227 en Limited Access Wiley Animal Genetics;24: 125-127 |
| spellingShingle | cattle genes Kemp, Stephen J. Maillard, J.C. Teale, A.J. A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title | A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title_full | A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title_fullStr | A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title_full_unstemmed | A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title_short | A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification |
| title_sort | polymorphism in the bovine gamma s crystallin gene revealed by allele specific amplification |
| topic | cattle genes |
| url | https://hdl.handle.net/10568/28227 |
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