Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa

Fungal infection and aflatoxin contamination was evaluated on 114 samples of dried and milled spices such as ginger, garlic and black pepper from southern Benin and Togo collected in November 2008-January 2009. These products are dried to preserve them for lean periods available throughout the year....

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Autores principales: Gnonlonfin, G.J.B., Adjovi, Y.C.S., Tokpo, A.F., Agbekponou, E.D., Ameyapoh, Y., Souza, C. de, Brimer, L., Sanni, A.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://hdl.handle.net/10568/27947
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author Gnonlonfin, G.J.B.
Adjovi, Y.C.S.
Tokpo, A.F.
Agbekponou, E.D.
Ameyapoh, Y.
Souza, C. de
Brimer, L.
Sanni, A.
author_browse Adjovi, Y.C.S.
Agbekponou, E.D.
Ameyapoh, Y.
Brimer, L.
Gnonlonfin, G.J.B.
Sanni, A.
Souza, C. de
Tokpo, A.F.
author_facet Gnonlonfin, G.J.B.
Adjovi, Y.C.S.
Tokpo, A.F.
Agbekponou, E.D.
Ameyapoh, Y.
Souza, C. de
Brimer, L.
Sanni, A.
author_sort Gnonlonfin, G.J.B.
collection Repository of Agricultural Research Outputs (CGSpace)
description Fungal infection and aflatoxin contamination was evaluated on 114 samples of dried and milled spices such as ginger, garlic and black pepper from southern Benin and Togo collected in November 2008-January 2009. These products are dried to preserve them for lean periods available throughout the year. Fungal contamination was evaluated after plating on selective media with a total of 20 fungal genera identified, ranging from 7 in garlic to 14 in ginger. Ginger and pepper showed high incidence of fungal contamination compared to garlic that had lower levels of fungal contamination. Species of Aspergillus were dominant on all marketed dried and milled spices irrespective of country. Gene characterization and amplification analysis showed that most of the A. flavus isolates possesses the cluster genes for aflatoxin production. Aflatoxin B1 assessment by Thin Layer Chromatography showed that only garlic (1 sample) and ginger (4 samples) were naturally contaminated with aflatoxin B1 ranging from 390 μg/kg to 1045 μg/kg respectively. Previous reports have mostly highlighted the risk of mycotoxin exposure from staple crops and vegetables in Africa, but such risks now need to be evaluated further for other products such as dried and milled spices.
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spelling CGSpace279472023-12-08T19:36:04Z Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa Gnonlonfin, G.J.B. Adjovi, Y.C.S. Tokpo, A.F. Agbekponou, E.D. Ameyapoh, Y. Souza, C. de Brimer, L. Sanni, A. research agriculture Fungal infection and aflatoxin contamination was evaluated on 114 samples of dried and milled spices such as ginger, garlic and black pepper from southern Benin and Togo collected in November 2008-January 2009. These products are dried to preserve them for lean periods available throughout the year. Fungal contamination was evaluated after plating on selective media with a total of 20 fungal genera identified, ranging from 7 in garlic to 14 in ginger. Ginger and pepper showed high incidence of fungal contamination compared to garlic that had lower levels of fungal contamination. Species of Aspergillus were dominant on all marketed dried and milled spices irrespective of country. Gene characterization and amplification analysis showed that most of the A. flavus isolates possesses the cluster genes for aflatoxin production. Aflatoxin B1 assessment by Thin Layer Chromatography showed that only garlic (1 sample) and ginger (4 samples) were naturally contaminated with aflatoxin B1 ranging from 390 μg/kg to 1045 μg/kg respectively. Previous reports have mostly highlighted the risk of mycotoxin exposure from staple crops and vegetables in Africa, but such risks now need to be evaluated further for other products such as dried and milled spices. 2013-11 2013-04-25T20:51:39Z 2013-04-25T20:51:39Z Journal Article https://hdl.handle.net/10568/27947 en Limited Access Elsevier Gnonlonfin, G.J.B., Adjovi, Y.C., Tokpo, A.F., Agbekponou, E.D., Ameyapoh, Y., Souza, C. de, Brimer, L. and Sanni, A. 2013. Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa. Food Control 34(1): 115-120.
spellingShingle research
agriculture
Gnonlonfin, G.J.B.
Adjovi, Y.C.S.
Tokpo, A.F.
Agbekponou, E.D.
Ameyapoh, Y.
Souza, C. de
Brimer, L.
Sanni, A.
Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title_full Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title_fullStr Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title_full_unstemmed Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title_short Mycobiota and identification of aflatoxin gene cluster in marketed spices in West Africa
title_sort mycobiota and identification of aflatoxin gene cluster in marketed spices in west africa
topic research
agriculture
url https://hdl.handle.net/10568/27947
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