A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon
Simple in vitro techniques were developed for collecting Digitaria eriantha ssp. pentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface- sterilisati...
| Main Author: | |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1991
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/27929 |
| _version_ | 1855538918103973888 |
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| author | Ruredzo, T.J. |
| author_browse | Ruredzo, T.J. |
| author_facet | Ruredzo, T.J. |
| author_sort | Ruredzo, T.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Simple in vitro techniques were developed for collecting Digitaria eriantha ssp. pentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface- sterilisation of plant material and for supplementing the growth medium to reduce microbial contamination. Halazon tablets were used for surface-sterilisation and a combination of Rifamycine and Benlate was the most effective medium supplement. This technique was found useful for the field collection of vegetative germplasm of the 2 species. In field trials, 75% of D. eriantha spp. pentzii and C. dactylon cultures were aseptic and growing after 4 weeks in culture. The cultures were transferred to natural conditions in soil and approximately 70% of the 2 species were recovered as potted plants. In a field trial with C. dactylon, 92% of the cultures which had formed roots and 80% of the cultures which had no roots at the time of transfer to soil were successfully established as potted plants. |
| format | Journal Article |
| id | CGSpace27929 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| record_format | dspace |
| spelling | CGSpace279292025-11-12T04:30:49Z A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon Ruredzo, T.J. feeds livestock animal feeding Simple in vitro techniques were developed for collecting Digitaria eriantha ssp. pentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface- sterilisation of plant material and for supplementing the growth medium to reduce microbial contamination. Halazon tablets were used for surface-sterilisation and a combination of Rifamycine and Benlate was the most effective medium supplement. This technique was found useful for the field collection of vegetative germplasm of the 2 species. In field trials, 75% of D. eriantha spp. pentzii and C. dactylon cultures were aseptic and growing after 4 weeks in culture. The cultures were transferred to natural conditions in soil and approximately 70% of the 2 species were recovered as potted plants. In a field trial with C. dactylon, 92% of the cultures which had formed roots and 80% of the cultures which had no roots at the time of transfer to soil were successfully established as potted plants. 1991 2013-04-22T08:15:36Z 2013-04-22T08:15:36Z Journal Article https://hdl.handle.net/10568/27929 en Open Access application/pdf Tropical Grasslands;25(1): 56-63 |
| spellingShingle | feeds livestock animal feeding Ruredzo, T.J. A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title | A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title_full | A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title_fullStr | A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title_full_unstemmed | A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title_short | A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon |
| title_sort | minimum facility method for in vitro collection of digitaria eriantha ssp pentzii and cynodon dactylon |
| topic | feeds livestock animal feeding |
| url | https://hdl.handle.net/10568/27929 |
| work_keys_str_mv | AT ruredzotj aminimumfacilitymethodforinvitrocollectionofdigitariaerianthassppentziiandcynodondactylon AT ruredzotj minimumfacilitymethodforinvitrocollectionofdigitariaerianthassppentziiandcynodondactylon |