A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon
Simple in vitro techniques were developed for collecting Digitaria eriantha sspentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface-sterilization o...
| Autor principal: | |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1991
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/27824 |
| _version_ | 1855542738881085440 |
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| author | Ruredzo, T.J. |
| author_browse | Ruredzo, T.J. |
| author_facet | Ruredzo, T.J. |
| author_sort | Ruredzo, T.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Simple in vitro techniques were developed for collecting Digitaria eriantha sspentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface-sterilization of plant material and for microbial contamination. Halazon tablets were used for surface-sterilization and a combination of Rifamycine and Benlate was the most effective medium supplement. Includes data on the effect of different combinations of antimicrobial agents on the performance of in vitro cultures of D. eriantha and performance of in vitro cultures and resulting potted plants as well as growth parameters of in vitro cultures and of potted plants. |
| format | Journal Article |
| id | CGSpace27824 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| record_format | dspace |
| spelling | CGSpace278242025-11-12T04:31:50Z A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon Ruredzo, T.J. digitaria eriantha cynodon dactylon plant collections methods culture media plant growth substances microorganisms sterilization Simple in vitro techniques were developed for collecting Digitaria eriantha sspentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface-sterilization of plant material and for microbial contamination. Halazon tablets were used for surface-sterilization and a combination of Rifamycine and Benlate was the most effective medium supplement. Includes data on the effect of different combinations of antimicrobial agents on the performance of in vitro cultures of D. eriantha and performance of in vitro cultures and resulting potted plants as well as growth parameters of in vitro cultures and of potted plants. 1991 2013-04-03T11:38:01Z 2013-04-03T11:38:01Z Journal Article https://hdl.handle.net/10568/27824 en Open Access application/pdf Tropical Grasslands;25(1):56-63 |
| spellingShingle | digitaria eriantha cynodon dactylon plant collections methods culture media plant growth substances microorganisms sterilization Ruredzo, T.J. A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title | A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title_full | A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title_fullStr | A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title_full_unstemmed | A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title_short | A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon |
| title_sort | minimum facility method for in vitro collection of digitaria eriantha sspentzii and cynodon dactylon |
| topic | digitaria eriantha cynodon dactylon plant collections methods culture media plant growth substances microorganisms sterilization |
| url | https://hdl.handle.net/10568/27824 |
| work_keys_str_mv | AT ruredzotj aminimumfacilitymethodforinvitrocollectionofdigitariaerianthasspentziiandcynodondactylon AT ruredzotj minimumfacilitymethodforinvitrocollectionofdigitariaerianthasspentziiandcynodondactylon |