Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia

Filed samples and seedlings of 51 leguminous forage species were tested for alfalfa mosaic virus (AMV) in routine monitoring of seed multiplication fields of the International Livestock Research Institute (ILRI), Ethiopia. The virus was detected in 24 species and a solanaceous weed Solanum nigrum. O...

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Main Authors: Mih, A.M., Hanson, Jean
Format: Journal Article
Language:Inglés
Published: 1998
Subjects:
Online Access:https://hdl.handle.net/10568/27813
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author Mih, A.M.
Hanson, Jean
author_browse Hanson, Jean
Mih, A.M.
author_facet Mih, A.M.
Hanson, Jean
author_sort Mih, A.M.
collection Repository of Agricultural Research Outputs (CGSpace)
description Filed samples and seedlings of 51 leguminous forage species were tested for alfalfa mosaic virus (AMV) in routine monitoring of seed multiplication fields of the International Livestock Research Institute (ILRI), Ethiopia. The virus was detected in 24 species and a solanaceous weed Solanum nigrum. Of these, the following 17 species are being reported for the first time as hosts of the virus in world literature: Aeschynomene falcata, Centrosema pascuorum, Chamaecrista rotundifolia, Desmanthus virgatus, Desmodium intortum, Leucaena leucocephala, Macroptilium atropurpureum, Macroptilium lathyroides, Macrotyloma axillare, Medicago truncatula, Neonotonia wightii, Sesbania sesban, Stylosanthes scabra, Trifolium calocephala, T. steudneri, T. tembense and Vigna parkeri. The solanaceous weed S. nigrum was suspected to play a role in the virus epidemiology. The level of seed infection varied from 0.00 percent to about 13.00 percent. Infectivity studies on 5 isolates of the virus revealed heterogeneity in prevalent AMV isolates. Two major strains were identified, based on the development of necrotic local lesions on Vigna unguiculata accessions. One of the isolates which failed to incite local necrotic lesions on unguiculata infected Phaseolus vulgaris cTop Crop systematically. Differences among isolates were also noticed in their concentration in different hosts as indicated by ELISA absorbance values.
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spelling CGSpace278132025-11-12T04:31:21Z Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia Mih, A.M. Hanson, Jean feed legumes plant viruses lucerne isolation techniques seed quality Filed samples and seedlings of 51 leguminous forage species were tested for alfalfa mosaic virus (AMV) in routine monitoring of seed multiplication fields of the International Livestock Research Institute (ILRI), Ethiopia. The virus was detected in 24 species and a solanaceous weed Solanum nigrum. Of these, the following 17 species are being reported for the first time as hosts of the virus in world literature: Aeschynomene falcata, Centrosema pascuorum, Chamaecrista rotundifolia, Desmanthus virgatus, Desmodium intortum, Leucaena leucocephala, Macroptilium atropurpureum, Macroptilium lathyroides, Macrotyloma axillare, Medicago truncatula, Neonotonia wightii, Sesbania sesban, Stylosanthes scabra, Trifolium calocephala, T. steudneri, T. tembense and Vigna parkeri. The solanaceous weed S. nigrum was suspected to play a role in the virus epidemiology. The level of seed infection varied from 0.00 percent to about 13.00 percent. Infectivity studies on 5 isolates of the virus revealed heterogeneity in prevalent AMV isolates. Two major strains were identified, based on the development of necrotic local lesions on Vigna unguiculata accessions. One of the isolates which failed to incite local necrotic lesions on unguiculata infected Phaseolus vulgaris cTop Crop systematically. Differences among isolates were also noticed in their concentration in different hosts as indicated by ELISA absorbance values. 1998 2013-04-03T11:37:59Z 2013-04-03T11:37:59Z Journal Article https://hdl.handle.net/10568/27813 en Open Access application/pdf Tropical Grasslands;32(2): 118-123
spellingShingle feed legumes
plant viruses
lucerne
isolation techniques
seed
quality
Mih, A.M.
Hanson, Jean
Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title_full Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title_fullStr Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title_full_unstemmed Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title_short Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia
title_sort alfalfa mosaic virus occurrence and variation among isolates from forage legumes in ethiopia
topic feed legumes
plant viruses
lucerne
isolation techniques
seed
quality
url https://hdl.handle.net/10568/27813
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