Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis
Chloris roxburghiana is an important rangeland grass in Kenya. In some areas it has disappeared due to land degradation resulting from overgrazing and drought. Efforts to re-introduce the grass through re-seeding using seeds from research stations have had little success. One possible reason for low...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2005
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/27697 |
| _version_ | 1855540915331923968 |
|---|---|
| author | Mnene, W.N. Hanson, Jean Ekaya, Wellington N. Kinyamario, J.I. Mweki, P. Lall, G. Stuth, J.W. Jamnadass, Ramni H. |
| author_browse | Ekaya, Wellington N. Hanson, Jean Jamnadass, Ramni H. Kinyamario, J.I. Lall, G. Mnene, W.N. Mweki, P. Stuth, J.W. |
| author_facet | Mnene, W.N. Hanson, Jean Ekaya, Wellington N. Kinyamario, J.I. Mweki, P. Lall, G. Stuth, J.W. Jamnadass, Ramni H. |
| author_sort | Mnene, W.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Chloris roxburghiana is an important rangeland grass in Kenya. In some areas it has disappeared due to land degradation resulting from overgrazing and drought. Efforts to re-introduce the grass through re-seeding using seeds from research stations have had little success. One possible reason for low establishment is attributed to transplanting spatially separated populations that may represent genetically distinct ecotypes. To test this hypothesis, germplasm diversity within and among four populations of C. roxburghiana from four ecologically distinct sites was analysed using random amplified polymorphic DNA (RAPD) markers. A total of 131 polymorphic markers were identified using nine RAPD primers. There was significant variation among populations, with genetic diversity (H) ranging from 0.134 to 0.206. Analysis of molecular variance (AMOVA) revealed most variance within populations (76%). However, the variation among populations was also significant (24%). The UPGMA of the population frequency indicated that the four populations of C. roxburghiana were genetically distinct, probably as a result of variation in soil fertility, geographical isolation and socio-ecological history of the study sites. Although there are differences for neutral genetic markers between the populations, it is difficult to relate this directly to ecotype since correlation does not imply causality. The implication for optimising future seed collection is discussed and potential areas for further studies identified. |
| format | Journal Article |
| id | CGSpace27697 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| record_format | dspace |
| spelling | CGSpace276972022-01-29T16:27:47Z Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis Mnene, W.N. Hanson, Jean Ekaya, Wellington N. Kinyamario, J.I. Mweki, P. Lall, G. Stuth, J.W. Jamnadass, Ramni H. forage Chloris roxburghiana is an important rangeland grass in Kenya. In some areas it has disappeared due to land degradation resulting from overgrazing and drought. Efforts to re-introduce the grass through re-seeding using seeds from research stations have had little success. One possible reason for low establishment is attributed to transplanting spatially separated populations that may represent genetically distinct ecotypes. To test this hypothesis, germplasm diversity within and among four populations of C. roxburghiana from four ecologically distinct sites was analysed using random amplified polymorphic DNA (RAPD) markers. A total of 131 polymorphic markers were identified using nine RAPD primers. There was significant variation among populations, with genetic diversity (H) ranging from 0.134 to 0.206. Analysis of molecular variance (AMOVA) revealed most variance within populations (76%). However, the variation among populations was also significant (24%). The UPGMA of the population frequency indicated that the four populations of C. roxburghiana were genetically distinct, probably as a result of variation in soil fertility, geographical isolation and socio-ecological history of the study sites. Although there are differences for neutral genetic markers between the populations, it is difficult to relate this directly to ecotype since correlation does not imply causality. The implication for optimising future seed collection is discussed and potential areas for further studies identified. 2005 2013-03-10T11:53:30Z 2013-03-10T11:53:30Z Journal Article https://hdl.handle.net/10568/27697 en Limited Access Mnene, W.N., Hanson, J., Ekaya, W.N., Kinyamario, J.I., Mweki, P., Lall, G., Stuth, J.W. and Jamnadass, R.H. 2005. Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis. African Journal of Range and Forage Science, 22(2): 107-115. |
| spellingShingle | forage Mnene, W.N. Hanson, Jean Ekaya, Wellington N. Kinyamario, J.I. Mweki, P. Lall, G. Stuth, J.W. Jamnadass, Ramni H. Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title | Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title_full | Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title_fullStr | Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title_full_unstemmed | Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title_short | Genetic variation between ecotypic populations of Chloris roxburghiana grass detected through RAPD analysis |
| title_sort | genetic variation between ecotypic populations of chloris roxburghiana grass detected through rapd analysis |
| topic | forage |
| url | https://hdl.handle.net/10568/27697 |
| work_keys_str_mv | AT mnenewn geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT hansonjean geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT ekayawellingtonn geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT kinyamarioji geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT mwekip geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT lallg geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT stuthjw geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis AT jamnadassramnih geneticvariationbetweenecotypicpopulationsofchlorisroxburghianagrassdetectedthroughrapdanalysis |