Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment
Reliable, sensitive, quantitative, and mobile rapid screening methods for pathogenic organisms are not yet readily available, but would provide a great benefit to humanitarian intervention units in disaster situations. We compared three different methods (immunofluorescent microscopy, IFM; flow cyto...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Chemical Society
2012
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/24645 |
| _version_ | 1855526821908447232 |
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| author | Keserue, H.A. Fuchslin, H.P. Wittwer, M. Hung Nguyen-Viet Nguyen, T.T. Surinkul, N. Koottatep, T. Schurch, N. Egli, T. |
| author_browse | Egli, T. Fuchslin, H.P. Hung Nguyen-Viet Keserue, H.A. Koottatep, T. Nguyen, T.T. Schurch, N. Surinkul, N. Wittwer, M. |
| author_facet | Keserue, H.A. Fuchslin, H.P. Wittwer, M. Hung Nguyen-Viet Nguyen, T.T. Surinkul, N. Koottatep, T. Schurch, N. Egli, T. |
| author_sort | Keserue, H.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Reliable, sensitive, quantitative, and mobile rapid screening methods for pathogenic organisms are not yet readily available, but would provide a great benefit to humanitarian intervention units in disaster situations. We compared three different methods (immunofluorescent microscopy, IFM; flow cytometry, FCM; polymerase chain reaction, PCR) for the rapid and quantitative detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in a field campaign. For this we deployed our mobile instrumentation and sampled canal water and vegetables during a 2 week field study in Thailand. For purification and concentrations of (oo)cysts, we used filtration and immunomagnetic separation. We were able to detect considerably high oo(cysts) concentrations (ranges: 15–855 and 0–240 oo(cysts)/liter for Giardia and Cryptosporidium, respectively) in 85 to 300 min, with FCM being fastest, followed by PCR, and IFM being slowest due to the long analysis time per sample. FCM and IFM performed consistently well, whereas PCR reactions often failed. The recovery, established by FCM, was around 30% for Giardia and 13% for Cryptosporidium (oo)cysts. It was possible to track (oo)cysts from the wastewater further downstream to irrigation waters and confirm contamination of salads and water vegetables. We believe that rapid detection, in particular FCM-based methods, can substantially help in disaster management and outbreak prevention. |
| format | Journal Article |
| id | CGSpace24645 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | CGSpace246452024-01-08T18:54:14Z Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment Keserue, H.A. Fuchslin, H.P. Wittwer, M. Hung Nguyen-Viet Nguyen, T.T. Surinkul, N. Koottatep, T. Schurch, N. Egli, T. food safety health Reliable, sensitive, quantitative, and mobile rapid screening methods for pathogenic organisms are not yet readily available, but would provide a great benefit to humanitarian intervention units in disaster situations. We compared three different methods (immunofluorescent microscopy, IFM; flow cytometry, FCM; polymerase chain reaction, PCR) for the rapid and quantitative detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in a field campaign. For this we deployed our mobile instrumentation and sampled canal water and vegetables during a 2 week field study in Thailand. For purification and concentrations of (oo)cysts, we used filtration and immunomagnetic separation. We were able to detect considerably high oo(cysts) concentrations (ranges: 15–855 and 0–240 oo(cysts)/liter for Giardia and Cryptosporidium, respectively) in 85 to 300 min, with FCM being fastest, followed by PCR, and IFM being slowest due to the long analysis time per sample. FCM and IFM performed consistently well, whereas PCR reactions often failed. The recovery, established by FCM, was around 30% for Giardia and 13% for Cryptosporidium (oo)cysts. It was possible to track (oo)cysts from the wastewater further downstream to irrigation waters and confirm contamination of salads and water vegetables. We believe that rapid detection, in particular FCM-based methods, can substantially help in disaster management and outbreak prevention. 2012-08-21 2012-10-29T20:07:10Z 2012-10-29T20:07:10Z Journal Article https://hdl.handle.net/10568/24645 en Limited Access American Chemical Society Keserue, H.-A.; Fuchslin, H.P.; Wittwer, M.; Hung Nguyen-Viet; Nguyen, T.T.; Surinkul, N.; Koottatep, T.; Schurch, N. and Egli, T. 2012. Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (Oo)cysts using transportable instrumentation in a field deployment. Environmental Science and Technology 46(16):8952-8959. |
| spellingShingle | food safety health Keserue, H.A. Fuchslin, H.P. Wittwer, M. Hung Nguyen-Viet Nguyen, T.T. Surinkul, N. Koottatep, T. Schurch, N. Egli, T. Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title | Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title_full | Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title_fullStr | Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title_full_unstemmed | Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title_short | Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment |
| title_sort | comparison of rapid methods for detection of giardia spp and cryptosporidium spp oo cysts using transportable instrumentation in a field deployment |
| topic | food safety health |
| url | https://hdl.handle.net/10568/24645 |
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