Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus

The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequen...

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Detalles Bibliográficos
Autores principales: Guerrero, F.D., Moolhuijzen, P., Peterson, D.G., Bidwell, S., Caler, E., Bellgard, M., Nene, Vishvanath M., Djikeng, Appolinaire
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2010
Materias:
Acceso en línea:https://hdl.handle.net/10568/2323
Descripción
Sumario:The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequencing.