Study on single nucleotide polymorphisms of H -FABP gene in sheep
PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48),...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Chinese |
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China Science Publishing & Media Ltd.
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/2168 |
| _version_ | 1855517238716530688 |
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| author | Qiao, H.Y. Zhao, Q.J. Yao, N. Lu, Y.-Z. Meng, X.-R. Guan, Wei-Jun Ma, Y.H. Han Jianlin |
| author_browse | Guan, Wei-Jun Han Jianlin Lu, Y.-Z. Ma, Y.H. Meng, X.-R. Qiao, H.Y. Yao, N. Zhao, Q.J. |
| author_facet | Qiao, H.Y. Zhao, Q.J. Yao, N. Lu, Y.-Z. Meng, X.-R. Guan, Wei-Jun Ma, Y.H. Han Jianlin |
| author_sort | Qiao, H.Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH＞Hh＞hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations. |
| format | Journal Article |
| id | CGSpace2168 |
| institution | CGIAR Consortium |
| language | Chinese |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | China Science Publishing & Media Ltd. |
| publisherStr | China Science Publishing & Media Ltd. |
| record_format | dspace |
| spelling | CGSpace21682024-04-25T06:00:26Z Study on single nucleotide polymorphisms of H -FABP gene in sheep Qiao, H.Y. Zhao, Q.J. Yao, N. Lu, Y.-Z. Meng, X.-R. Guan, Wei-Jun Ma, Y.H. Han Jianlin genetics sheep PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH＞Hh＞hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations. 2009-08-14 2010-08-04T13:29:08Z 2010-08-04T13:29:08Z Journal Article https://hdl.handle.net/10568/2168 zh Limited Access China Science Publishing & Media Ltd. Qiao, H.-Y., Zhao, Q.-J., Yao, N., Lu, Y.-Z., Meng, X.-R., Jianlin, H., Guan, W.-J.; Ma, Y.-H. 2009. Study on single nucleotide polymorphisms of H -FABP gene in sheep. Hereditas (Beijing) 31(7):725-731. |
| spellingShingle | genetics sheep Qiao, H.Y. Zhao, Q.J. Yao, N. Lu, Y.-Z. Meng, X.-R. Guan, Wei-Jun Ma, Y.H. Han Jianlin Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title | Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title_full | Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title_fullStr | Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title_full_unstemmed | Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title_short | Study on single nucleotide polymorphisms of H -FABP gene in sheep |
| title_sort | study on single nucleotide polymorphisms of h fabp gene in sheep |
| topic | genetics sheep |
| url | https://hdl.handle.net/10568/2168 |
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