Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage
Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2005
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/21180 |
| _version_ | 1855522640799727616 |
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| author | Bishop, Richard P. Shah, Tushaar Pelle, Roger Hoyle, D. Pearson, T. Haines, L. Brass, A. Hulme, H. Graham, S.P. Taracha, E.L.N. Lu, C. Hass, B. Wortman, Jennifer R. White, O. Gardner, M.J. Nene, Vishvanath M. Villiers, Etienne P. de |
| author_browse | Bishop, Richard P. Brass, A. Gardner, M.J. Graham, S.P. Haines, L. Hass, B. Hoyle, D. Hulme, H. Lu, C. Nene, Vishvanath M. Pearson, T. Pelle, Roger Shah, Tushaar Taracha, E.L.N. Villiers, Etienne P. de White, O. Wortman, Jennifer R. |
| author_facet | Bishop, Richard P. Shah, Tushaar Pelle, Roger Hoyle, D. Pearson, T. Haines, L. Brass, A. Hulme, H. Graham, S.P. Taracha, E.L.N. Lu, C. Hass, B. Wortman, Jennifer R. White, O. Gardner, M.J. Nene, Vishvanath M. Villiers, Etienne P. de |
| author_sort | Bishop, Richard P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4–52 256 transcripts per million (t.p.m.). Rare transcripts (<50 t.p.m.) were detected from 36% of genes. Sequence signatures approximated a lognormal distribution, as in microarray. Transcripts were widely distributed throughout the genome, although only 47% of 138 telomere-associated open reading frames exhibited signatures. Antisense signatures comprised 13.8% of the total, comparable with Plasmodium. Eighty five predicted genes with antisense signatures lacked a sense signature. Antisense transcripts were independently amplified from schizont cDNA and verified by sequencing. The MPSS transcripts per million for seven genes encoding schizont antigens recognized by bovine CD8 T cells varied 1000-fold. There was concordance between transcription and protein expression for heat shock proteins that were very highly expressed according to MPSS and proteomics. The data suggests a low level of baseline transcription from the majority of protein-coding genes. |
| format | Journal Article |
| id | CGSpace21180 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace211802025-11-13T10:38:43Z Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage Bishop, Richard P. Shah, Tushaar Pelle, Roger Hoyle, D. Pearson, T. Haines, L. Brass, A. Hulme, H. Graham, S.P. Taracha, E.L.N. Lu, C. Hass, B. Wortman, Jennifer R. White, O. Gardner, M.J. Nene, Vishvanath M. Villiers, Etienne P. de theileria parva genetics Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4–52 256 transcripts per million (t.p.m.). Rare transcripts (<50 t.p.m.) were detected from 36% of genes. Sequence signatures approximated a lognormal distribution, as in microarray. Transcripts were widely distributed throughout the genome, although only 47% of 138 telomere-associated open reading frames exhibited signatures. Antisense signatures comprised 13.8% of the total, comparable with Plasmodium. Eighty five predicted genes with antisense signatures lacked a sense signature. Antisense transcripts were independently amplified from schizont cDNA and verified by sequencing. The MPSS transcripts per million for seven genes encoding schizont antigens recognized by bovine CD8 T cells varied 1000-fold. There was concordance between transcription and protein expression for heat shock proteins that were very highly expressed according to MPSS and proteomics. The data suggests a low level of baseline transcription from the majority of protein-coding genes. 2005-09-25 2012-07-09T05:59:45Z 2012-07-09T05:59:45Z Journal Article https://hdl.handle.net/10568/21180 en Open Access Oxford University Press Bishop, R., Shah, T., Pellé, R., Hoyle, D., Pearson, T., Haines, L., Brass, A., Hulme, H., Graham, S.P., Taracha, E.L.N., Lu, C., Hass, B., Wortman, J., White, O., Gardner, M.J., Nene, V. and Villiers, E.P. de 2005. Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage. Nucleic Acids Research 33(17): 5503-5511. |
| spellingShingle | theileria parva genetics Bishop, Richard P. Shah, Tushaar Pelle, Roger Hoyle, D. Pearson, T. Haines, L. Brass, A. Hulme, H. Graham, S.P. Taracha, E.L.N. Lu, C. Hass, B. Wortman, Jennifer R. White, O. Gardner, M.J. Nene, Vishvanath M. Villiers, Etienne P. de Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title | Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title_full | Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title_fullStr | Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title_full_unstemmed | Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title_short | Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage |
| title_sort | analysis of the transcriptome of the protozoan theileria parva using mpss reveals that the majority of genes are transcriptionally active in the schizont stage |
| topic | theileria parva genetics |
| url | https://hdl.handle.net/10568/21180 |
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