Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses

The pan-tropical grass genus Brachiaria comprises about 100 species, several of which are forages of economic importance, particularly in tropical America. Acremonium implicatum is a fungus that forms an endophytic association with at least some of these economically important grasses. To ascertain...

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Autores principales: Dongyi, H., Kelemu, Segenet
Formato: Journal Article
Lenguaje:Inglés
Publicado: Scientific Societies 2004
Materias:
Acceso en línea:https://hdl.handle.net/10568/21175
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author Dongyi, H.
Kelemu, Segenet
author_browse Dongyi, H.
Kelemu, Segenet
author_facet Dongyi, H.
Kelemu, Segenet
author_sort Dongyi, H.
collection Repository of Agricultural Research Outputs (CGSpace)
description The pan-tropical grass genus Brachiaria comprises about 100 species, several of which are forages of economic importance, particularly in tropical America. Acremonium implicatum is a fungus that forms an endophytic association with at least some of these economically important grasses. To ascertain whether A. implicatum can be seed transmitted in Brachiaria species, we vegetatively propagated, under greenhouse conditions, 20 tillers from an endophyte-infected mother plant obtained from each of 14 Brachiaria hybrids and species. Ten tillers of each genotype were treated with the fungicide tebuconazole to eliminate the endophyte, and the other 10 were left untreated. Seeds were then harvested individually from all 20 of these genetically identical plants, germinated, and the seedlings grown. A previously developed polymerase chain reaction-based method used a pair of endophyte-specific primers to amplify a diagnostic 500-bp DNA fragment. The seedlings generated from seeds harvested from endophyte-infected plants also tested positive, whereas those from seeds of endophyte-free plants showed no amplification products. This is the first report of A. implicatum being transmitted through seeds of Brachiaria grasses.
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spelling CGSpace211752025-06-13T04:20:13Z Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses Dongyi, H. Kelemu, Segenet grasses plant diseases feed crops brachiaria fungal diseases seedborne organisms apomixis acremonium implicatum pcr enfermedades fungosas organismos transmitidos por semilla The pan-tropical grass genus Brachiaria comprises about 100 species, several of which are forages of economic importance, particularly in tropical America. Acremonium implicatum is a fungus that forms an endophytic association with at least some of these economically important grasses. To ascertain whether A. implicatum can be seed transmitted in Brachiaria species, we vegetatively propagated, under greenhouse conditions, 20 tillers from an endophyte-infected mother plant obtained from each of 14 Brachiaria hybrids and species. Ten tillers of each genotype were treated with the fungicide tebuconazole to eliminate the endophyte, and the other 10 were left untreated. Seeds were then harvested individually from all 20 of these genetically identical plants, germinated, and the seedlings grown. A previously developed polymerase chain reaction-based method used a pair of endophyte-specific primers to amplify a diagnostic 500-bp DNA fragment. The seedlings generated from seeds harvested from endophyte-infected plants also tested positive, whereas those from seeds of endophyte-free plants showed no amplification products. This is the first report of A. implicatum being transmitted through seeds of Brachiaria grasses. 2004-11 2012-07-09T05:52:02Z 2012-07-09T05:52:02Z Journal Article https://hdl.handle.net/10568/21175 en Open Access Scientific Societies Dongyi, H., & Kelemu, S. (2004). Acremonium implicatum, a Seed-Transmitted Endophytic Fungus in Brachiaria Grasses. Plant Disease, 88(11), 1252–1254. https://doi.org/10.1094/pdis.2004.88.11.1252
spellingShingle grasses
plant diseases
feed crops
brachiaria
fungal diseases
seedborne organisms
apomixis
acremonium implicatum
pcr
enfermedades fungosas
organismos transmitidos por semilla
Dongyi, H.
Kelemu, Segenet
Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title_full Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title_fullStr Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title_full_unstemmed Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title_short Acremonium implicatum, a seed-transmitted endophytic fungus in Brachiaria grasses
title_sort acremonium implicatum a seed transmitted endophytic fungus in brachiaria grasses
topic grasses
plant diseases
feed crops
brachiaria
fungal diseases
seedborne organisms
apomixis
acremonium implicatum
pcr
enfermedades fungosas
organismos transmitidos por semilla
url https://hdl.handle.net/10568/21175
work_keys_str_mv AT dongyih acremoniumimplicatumaseedtransmittedendophyticfungusinbrachiariagrasses
AT kelemusegenet acremoniumimplicatumaseedtransmittedendophyticfungusinbrachiariagrasses