Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from ticks...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2004
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/21173 |
| _version_ | 1855543076987076608 |
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| author | Nene, Vishvanath M. Lee, D. Kang'a, S. Skilton, Robert A. Shah, Tushaar Villiers, Etienne P. de Mwaura, S. Taylor, D. Quackenbush, J. Bishop, Richard P. |
| author_browse | Bishop, Richard P. Kang'a, S. Lee, D. Mwaura, S. Nene, Vishvanath M. Quackenbush, J. Shah, Tushaar Skilton, Robert A. Taylor, D. Villiers, Etienne P. de |
| author_facet | Nene, Vishvanath M. Lee, D. Kang'a, S. Skilton, Robert A. Shah, Tushaar Villiers, Etienne P. de Mwaura, S. Taylor, D. Quackenbush, J. Bishop, Richard P. |
| author_sort | Nene, Vishvanath M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from ticks infected with Theileria parva, which develop in type III salivary gland acini. There were no major differences between abundantly expressed ESTs from the two cDNA libraries, although there was evidence for an up-regulation in the expression of some glycine-rich proteins in infected salivary glands. Gene ontology terms were also assigned to sequences in the index and those with potential enzyme function were linked to the Kyoto encyclopedia of genes and genomes database, allowing reconstruction of metabolic pathways. Several genes code for previously characterized tick proteins such as receptors for myokinin or ecdysteroid and an immunosuppressive protein. cDNAs coding for homologs of heme-lipoproteins which are major components of tick hemolymph were identified by searching the database with published N-terminal peptide sequence data derived from biochemically purified Boophilus microplus proteins. The EST data will be a useful resource for construction of microarrays to probe vector biology, vector–host and vector–pathogen interactions and to underpin gene identification via proteomics approaches. |
| format | Journal Article |
| id | CGSpace21173 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace211732025-12-08T09:54:28Z Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva Nene, Vishvanath M. Lee, D. Kang'a, S. Skilton, Robert A. Shah, Tushaar Villiers, Etienne P. de Mwaura, S. Taylor, D. Quackenbush, J. Bishop, Richard P. animal health animal diseases ticks rhipicephalus appendiculatus metastigmata arthropoda genomes theileria parva transcription genes salivary glands [digestive system] We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from ticks infected with Theileria parva, which develop in type III salivary gland acini. There were no major differences between abundantly expressed ESTs from the two cDNA libraries, although there was evidence for an up-regulation in the expression of some glycine-rich proteins in infected salivary glands. Gene ontology terms were also assigned to sequences in the index and those with potential enzyme function were linked to the Kyoto encyclopedia of genes and genomes database, allowing reconstruction of metabolic pathways. Several genes code for previously characterized tick proteins such as receptors for myokinin or ecdysteroid and an immunosuppressive protein. cDNAs coding for homologs of heme-lipoproteins which are major components of tick hemolymph were identified by searching the database with published N-terminal peptide sequence data derived from biochemically purified Boophilus microplus proteins. The EST data will be a useful resource for construction of microarrays to probe vector biology, vector–host and vector–pathogen interactions and to underpin gene identification via proteomics approaches. 2004-10 2012-07-09T05:45:42Z 2012-07-09T05:45:42Z Journal Article https://hdl.handle.net/10568/21173 en Limited Access Elsevier Nene, V., Lee, D., Kang’a, S., Skilton, R., Shah, T., de Villiers, E., Mwaura, S., Taylor, D., Quackenbush, J., & Bishop, R. (2004). Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva. In Insect Biochemistry and Molecular Biology (Vol. 34, Issue 10, pp. 1117–1128). Elsevier BV. https://doi.org/10.1016/j.ibmb.2004.07.002 |
| spellingShingle | animal health animal diseases ticks rhipicephalus appendiculatus metastigmata arthropoda genomes theileria parva transcription genes salivary glands [digestive system] Nene, Vishvanath M. Lee, D. Kang'a, S. Skilton, Robert A. Shah, Tushaar Villiers, Etienne P. de Mwaura, S. Taylor, D. Quackenbush, J. Bishop, Richard P. Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title | Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title_full | Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title_fullStr | Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title_full_unstemmed | Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title_short | Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva |
| title_sort | genes transcribed in the salivary glands of female rhipicephalus appendiculatus ticks infected with theileria parva |
| topic | animal health animal diseases ticks rhipicephalus appendiculatus metastigmata arthropoda genomes theileria parva transcription genes salivary glands [digestive system] |
| url | https://hdl.handle.net/10568/21173 |
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