AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index ca...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2002
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/21172 |
| _version_ | 1855523524618223616 |
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| author | Nene, Vishvanath M. Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. |
| author_browse | Bishop, Richard P. Gardner, M.J. Lee, D. Mwaura, S. Nene, Vishvanath M. Quackenbush, J. Skilton, Robert A. |
| author_facet | Nene, Vishvanath M. Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. |
| author_sort | Nene, Vishvanath M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species. The database may be accessed via the World Wide Web at http://www.tigr.org/tdb/tgi.shtml. |
| format | Journal Article |
| id | CGSpace21172 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace211722025-06-13T04:20:13Z AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum Nene, Vishvanath M. Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. ticks parasites amblyomma variegatum genes salivary glands metastigmata dna Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species. The database may be accessed via the World Wide Web at http://www.tigr.org/tdb/tgi.shtml. 2002-11 2012-07-09T05:44:20Z 2012-07-09T05:44:20Z Journal Article https://hdl.handle.net/10568/21172 en Limited Access Elsevier Nene, V., Lee, D., Quackenbush, J., Skilton, R., Mwaura, S., Gardner, M. J., & Bishop, R. (2002). AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum. In International Journal for Parasitology (Vol. 32, Issue 12, pp. 1447–1456). Elsevier BV. https://doi.org/10.1016/s0020-7519(02)00159-5 |
| spellingShingle | ticks parasites amblyomma variegatum genes salivary glands metastigmata dna Nene, Vishvanath M. Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title | AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title_full | AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title_fullStr | AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title_full_unstemmed | AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title_short | AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
| title_sort | avgi an index of genes transcribed in the salivary glands of the ixodid tick amblyomma variegatum |
| topic | ticks parasites amblyomma variegatum genes salivary glands metastigmata dna |
| url | https://hdl.handle.net/10568/21172 |
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