Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques

Dung counts have been widely used to estimate duiker abundance and densities in tropical African forests. However, one of the major limitations of this method is that species’ identification of dung based on morphological characteristics is extremely difficult in most cases. Some authors made the as...

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Main Authors: Vliet, N. van, Zundel, S, Miguel, C., Taberlet, P, Nasi, Robert
Format: Journal Article
Language:Inglés
Published: Wiley 2008
Subjects:
Online Access:https://hdl.handle.net/10568/19935
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author Vliet, N. van
Zundel, S
Miguel, C.
Taberlet, P
Nasi, Robert
author_browse Miguel, C.
Nasi, Robert
Taberlet, P
Vliet, N. van
Zundel, S
author_facet Vliet, N. van
Zundel, S
Miguel, C.
Taberlet, P
Nasi, Robert
author_sort Vliet, N. van
collection Repository of Agricultural Research Outputs (CGSpace)
description Dung counts have been widely used to estimate duiker abundance and densities in tropical African forests. However, one of the major limitations of this method is that species’ identification of dung based on morphological characteristics is extremely difficult in most cases. Some authors made the assumption that dung pellets could at least be distinguishable between the blue duiker, the red duikers and the yellow-backed duiker. However, this study is the first attempt to test the reliability of field identification. In this study, we suggest a method based on a noninvasive genetic protocol to identify duiker species. The comparison of field and genetic identifications shows that only dung from Cephalophus silvicultor was identified in the field without error. The rate of error for red duikers and the blue duiker is high and any attempt to distinguish among those species will result in wrong estimations for each species. We recommend the use of DNA tests to ensure reliable species’ identification when duiker censuses based on dung counts are used. This methodological input will probably strengthen the dung count technique and increase its reliability for duiker species abundance estimations and spatial distribution studies.
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spelling CGSpace199352024-08-27T10:35:15Z Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques Vliet, N. van Zundel, S Miguel, C. Taberlet, P Nasi, Robert mitochondrial dna surveys population density population growth Dung counts have been widely used to estimate duiker abundance and densities in tropical African forests. However, one of the major limitations of this method is that species’ identification of dung based on morphological characteristics is extremely difficult in most cases. Some authors made the assumption that dung pellets could at least be distinguishable between the blue duiker, the red duikers and the yellow-backed duiker. However, this study is the first attempt to test the reliability of field identification. In this study, we suggest a method based on a noninvasive genetic protocol to identify duiker species. The comparison of field and genetic identifications shows that only dung from Cephalophus silvicultor was identified in the field without error. The rate of error for red duikers and the blue duiker is high and any attempt to distinguish among those species will result in wrong estimations for each species. We recommend the use of DNA tests to ensure reliable species’ identification when duiker censuses based on dung counts are used. This methodological input will probably strengthen the dung count technique and increase its reliability for duiker species abundance estimations and spatial distribution studies. 2008-09 2012-06-04T09:12:52Z 2012-06-04T09:12:52Z Journal Article https://hdl.handle.net/10568/19935 en Limited Access Wiley Van Vliet, N., Zundel,S, Miguel, C., Taberlet, P, Nasi, R. 2008. Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques . African Journal of Ecology 46 (3) :411-417 doi: 10.1111/j.1365-2028.2007.00879.x. ISSN: 0141-6707.
spellingShingle mitochondrial dna
surveys
population density
population growth
Vliet, N. van
Zundel, S
Miguel, C.
Taberlet, P
Nasi, Robert
Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title_full Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title_fullStr Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title_full_unstemmed Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title_short Distinguishing dung from blue, red and yellow-backed duikers through noninvasive genetic techniques
title_sort distinguishing dung from blue red and yellow backed duikers through noninvasive genetic techniques
topic mitochondrial dna
surveys
population density
population growth
url https://hdl.handle.net/10568/19935
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AT zundels distinguishingdungfromblueredandyellowbackedduikersthroughnoninvasivegenetictechniques
AT miguelc distinguishingdungfromblueredandyellowbackedduikersthroughnoninvasivegenetictechniques
AT taberletp distinguishingdungfromblueredandyellowbackedduikersthroughnoninvasivegenetictechniques
AT nasirobert distinguishingdungfromblueredandyellowbackedduikersthroughnoninvasivegenetictechniques