A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes
Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the transcription...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2010
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/1921 |
| _version_ | 1855542915917414400 |
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| author | Kyoko, H. Masakazu, H. Ryo, N. Yoshimasa, T. Jung-Yeon, K. Noboru, I. Nene, Vishvanath M. Chihiro, S. |
| author_browse | Chihiro, S. Jung-Yeon, K. Kyoko, H. Masakazu, H. Nene, Vishvanath M. Noboru, I. Ryo, N. Yoshimasa, T. |
| author_facet | Kyoko, H. Masakazu, H. Ryo, N. Yoshimasa, T. Jung-Yeon, K. Noboru, I. Nene, Vishvanath M. Chihiro, S. |
| author_sort | Kyoko, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the transcription factor NF-κB is activated in schizont–infected cells, which makes the T. parva-transformed cells resistant to apoptosis. However, the mechanism by which the parasite triggers the activation of NF-κB remains enigmatic. In the present study, we biochemically characterized a novel protein, which we termed TpSCOP, which is expressed in the schizont stage of T. parva. TpSCOP was shown to interact with F-actin in vitro. Expression of TpSCOP in a murine lymphocytic cell line resulted in the activation of NF-κB signaling pathways, leading to apoptosis resistance. The activation of MAPKs, including ERK and JNK, were also detected. Furthermore, the introduction of TpSCOP into T. parva-infected cells also enhanced the activation of NF-κB. This is the first report to demonstrate that parasite-derived molecule has the ability to activate the host NF-κB pathway. Based on these results, TpSCOP likely plays an important role in apoptosis inhibition during Theileria infection. |
| format | Journal Article |
| id | CGSpace1921 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace19212024-08-27T10:37:26Z A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes Kyoko, H. Masakazu, H. Ryo, N. Yoshimasa, T. Jung-Yeon, K. Noboru, I. Nene, Vishvanath M. Chihiro, S. theileria parva cattle Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the transcription factor NF-κB is activated in schizont–infected cells, which makes the T. parva-transformed cells resistant to apoptosis. However, the mechanism by which the parasite triggers the activation of NF-κB remains enigmatic. In the present study, we biochemically characterized a novel protein, which we termed TpSCOP, which is expressed in the schizont stage of T. parva. TpSCOP was shown to interact with F-actin in vitro. Expression of TpSCOP in a murine lymphocytic cell line resulted in the activation of NF-κB signaling pathways, leading to apoptosis resistance. The activation of MAPKs, including ERK and JNK, were also detected. Furthermore, the introduction of TpSCOP into T. parva-infected cells also enhanced the activation of NF-κB. This is the first report to demonstrate that parasite-derived molecule has the ability to activate the host NF-κB pathway. Based on these results, TpSCOP likely plays an important role in apoptosis inhibition during Theileria infection. 2010-11 2010-06-14T08:26:59Z 2010-06-14T08:26:59Z Journal Article https://hdl.handle.net/10568/1921 en Limited Access Elsevier Kyoko Hayashida; Masakazu Hattori; Ryo Nakao; Yoshimasa Tanaka; Jung-Yeon Kim; Noboru Inoue; Nene, V.; Chihiro Sugimoto. 2010. A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes. Molecular and Biochemical Parasitology |
| spellingShingle | theileria parva cattle Kyoko, H. Masakazu, H. Ryo, N. Yoshimasa, T. Jung-Yeon, K. Noboru, I. Nene, Vishvanath M. Chihiro, S. A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title | A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title_full | A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title_fullStr | A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title_full_unstemmed | A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title_short | A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes |
| title_sort | schizont derived protein tpscop is involved in the activation of nf κb in theileria parva infected lymphocytes |
| topic | theileria parva cattle |
| url | https://hdl.handle.net/10568/1921 |
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