Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti
In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evaluated...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/1873 |
| _version_ | 1855513352023834624 |
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| author | Jeong, W. Kweon, C.H. Kwang, S.W. Hu Suk Lee Xu, Y. Lu, C. Zhang, S. Nene, Vishvanath M. |
| author_browse | Hu Suk Lee Jeong, W. Kwang, S.W. Kweon, C.H. Lu, C. Nene, Vishvanath M. Xu, Y. Zhang, S. |
| author_facet | Jeong, W. Kweon, C.H. Kwang, S.W. Hu Suk Lee Xu, Y. Lu, C. Zhang, S. Nene, Vishvanath M. |
| author_sort | Jeong, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evaluated with regard to antibody response, cytokine production, and a challenge experiment in mice or cattle. HSP-p33 fusion protein provoked higher humoral and cellular immunity than either Escherichia coli-expressed p33 or piroplasm soluble protein. The HSP adjuvant activity toward p33 was also possible to detect in the inoculated cattle. The overall growth of parasites in cattle was significantly restrained in the HSP-p33-inoculated group, up to 50-52 days longer than in the controls. The present results indicate that HSP-p33 fusion protein is a promising candidate vaccine for clinical theileriosis in the field. |
| format | Journal Article |
| id | CGSpace1873 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace18732024-01-17T12:58:34Z Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti Jeong, W. Kweon, C.H. Kwang, S.W. Hu Suk Lee Xu, Y. Lu, C. Zhang, S. Nene, Vishvanath M. disease control theileria sergenti pharmacology bioengineering biotechnology In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evaluated with regard to antibody response, cytokine production, and a challenge experiment in mice or cattle. HSP-p33 fusion protein provoked higher humoral and cellular immunity than either Escherichia coli-expressed p33 or piroplasm soluble protein. The HSP adjuvant activity toward p33 was also possible to detect in the inoculated cattle. The overall growth of parasites in cattle was significantly restrained in the HSP-p33-inoculated group, up to 50-52 days longer than in the controls. The present results indicate that HSP-p33 fusion protein is a promising candidate vaccine for clinical theileriosis in the field. 2009-10 2010-06-13T16:12:38Z 2010-06-13T16:12:38Z Journal Article https://hdl.handle.net/10568/1873 en Limited Access Elsevier Jeong, W.; Kweon, C.H.; Kang, S.W.; Lee, H.S.; Xu, Y.; Lu, C.; Zhang, S.; Nene, V. 2009. Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein p33 of Theileria sergenti. Biologicals 37(5):282-287. |
| spellingShingle | disease control theileria sergenti pharmacology bioengineering biotechnology Jeong, W. Kweon, C.H. Kwang, S.W. Hu Suk Lee Xu, Y. Lu, C. Zhang, S. Nene, Vishvanath M. Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title | Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title_full | Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title_fullStr | Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title_full_unstemmed | Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title_short | Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti |
| title_sort | adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein p33 of theileria sergenti |
| topic | disease control theileria sergenti pharmacology bioengineering biotechnology |
| url | https://hdl.handle.net/10568/1873 |
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