Rapid Ragi: A speed breeding protocol for finger millet

Background Climate change is gradually increasing demand for resilient, nutritious crops like finger millet or ragi. Ensuring food security requires researchers to develop improved and adapted cultivars rapidly. Modern techniques such as genomics-assisted breeding have emerged in the previous decade...

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Autores principales: Sajja, Sobhan, Pranati, Jwala, Shyamala, S., Vinutha, K.S., Reddy, Ramya, Joshi, Priyanka, Pannem, Srinivasulu, Rakshit, P., Jadhav, Yashoda, Kumar, C.V. Sameer, Mayes, Sean
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/180415
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author Sajja, Sobhan
Pranati, Jwala
Shyamala, S.
Vinutha, K.S.
Reddy, Ramya
Joshi, Priyanka
Pannem, Srinivasulu
Rakshit, P.
Jadhav, Yashoda
Kumar, C.V. Sameer
Mayes, Sean
author_browse Jadhav, Yashoda
Joshi, Priyanka
Kumar, C.V. Sameer
Mayes, Sean
Pannem, Srinivasulu
Pranati, Jwala
Rakshit, P.
Reddy, Ramya
Sajja, Sobhan
Shyamala, S.
Vinutha, K.S.
author_facet Sajja, Sobhan
Pranati, Jwala
Shyamala, S.
Vinutha, K.S.
Reddy, Ramya
Joshi, Priyanka
Pannem, Srinivasulu
Rakshit, P.
Jadhav, Yashoda
Kumar, C.V. Sameer
Mayes, Sean
author_sort Sajja, Sobhan
collection Repository of Agricultural Research Outputs (CGSpace)
description Background Climate change is gradually increasing demand for resilient, nutritious crops like finger millet or ragi. Ensuring food security requires researchers to develop improved and adapted cultivars rapidly. Modern techniques such as genomics-assisted breeding have emerged in the previous decade and combined with rapid generation advancement they will offer a step change in the speed of cultivar development. Results In this study, we developed a repeatable and cost-effective speed breeding protocol for finger millet by modulating the agronomic and physiological components for early generation advancement. A photoperiod of 9-hours, 29 ± 2℃ temperature, 70% relative humidity, 105 plants per 1.5 sq. ft., 0.17% Hoagland’s No. 2 solution spray, restricted irrigation and harvesting at physiological maturity successfully reduced 28–54 days across the maturity groups of finger millet. The advantage was validated in segregating populations confirming up to 4–5 generations a year, instead of 1–2 under field conditions. Conclusion The speed breeding protocol developed reduces the breeding cycle time significantly allowing increased genetic gain. The protocol provides the advantage of rapid development of recombinant inbred lines (RILs), high-throughput phenotyping for biotic and abiotic stresses, and genotyping for early generation selections.
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spelling CGSpace1804152026-01-23T02:04:54Z Rapid Ragi: A speed breeding protocol for finger millet Sajja, Sobhan Pranati, Jwala Shyamala, S. Vinutha, K.S. Reddy, Ramya Joshi, Priyanka Pannem, Srinivasulu Rakshit, P. Jadhav, Yashoda Kumar, C.V. Sameer Mayes, Sean agricultural genetics genetic techniques quantitative trait marker assisted selection plant breeding reverse breeding Background Climate change is gradually increasing demand for resilient, nutritious crops like finger millet or ragi. Ensuring food security requires researchers to develop improved and adapted cultivars rapidly. Modern techniques such as genomics-assisted breeding have emerged in the previous decade and combined with rapid generation advancement they will offer a step change in the speed of cultivar development. Results In this study, we developed a repeatable and cost-effective speed breeding protocol for finger millet by modulating the agronomic and physiological components for early generation advancement. A photoperiod of 9-hours, 29 ± 2℃ temperature, 70% relative humidity, 105 plants per 1.5 sq. ft., 0.17% Hoagland’s No. 2 solution spray, restricted irrigation and harvesting at physiological maturity successfully reduced 28–54 days across the maturity groups of finger millet. The advantage was validated in segregating populations confirming up to 4–5 generations a year, instead of 1–2 under field conditions. Conclusion The speed breeding protocol developed reduces the breeding cycle time significantly allowing increased genetic gain. The protocol provides the advantage of rapid development of recombinant inbred lines (RILs), high-throughput phenotyping for biotic and abiotic stresses, and genotyping for early generation selections. 2025-06-12 2026-01-22T13:39:02Z 2026-01-22T13:39:02Z Journal Article https://hdl.handle.net/10568/180415 en Open Access application/pdf Springer Sajja, Sobhan; Pranati, Jwala; Shyamala, S.; Vinutha, K. S.; Reddy, Ramya; Joshi, Priyanka; Pannem, Srinivasulu; Rakshit, P.; Jadhav, Yashoda; Kumar, C. V. Sameer; & Mayes, Sean. 2025. Rapid Ragi: A speed breeding protocol for finger millet. Plant Method 21(84)https://doi.org/10.1186/s13007-025-01403-7
spellingShingle agricultural genetics
genetic techniques
quantitative trait
marker assisted selection
plant breeding
reverse breeding
Sajja, Sobhan
Pranati, Jwala
Shyamala, S.
Vinutha, K.S.
Reddy, Ramya
Joshi, Priyanka
Pannem, Srinivasulu
Rakshit, P.
Jadhav, Yashoda
Kumar, C.V. Sameer
Mayes, Sean
Rapid Ragi: A speed breeding protocol for finger millet
title Rapid Ragi: A speed breeding protocol for finger millet
title_full Rapid Ragi: A speed breeding protocol for finger millet
title_fullStr Rapid Ragi: A speed breeding protocol for finger millet
title_full_unstemmed Rapid Ragi: A speed breeding protocol for finger millet
title_short Rapid Ragi: A speed breeding protocol for finger millet
title_sort rapid ragi a speed breeding protocol for finger millet
topic agricultural genetics
genetic techniques
quantitative trait
marker assisted selection
plant breeding
reverse breeding
url https://hdl.handle.net/10568/180415
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