High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis
A high throughput in vitro screening method for phenotyping banana resistance to Radopholus similis was developed and validated using sand-MS media. The reproduction factor of R. similis showed a similar trend under in vitro and screenhouse conditions. The in vitro method proved sensitive and cost-e...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/180307 |
| _version_ | 1855531715777265664 |
|---|---|
| author | Kisitu, J. Saikai, K.K. Coyne, D. Kanaabi, R. Kisaakye, J. Swennen, R. Nakato, G.V. |
| author_browse | Coyne, D. Kanaabi, R. Kisaakye, J. Kisitu, J. Nakato, G.V. Saikai, K.K. Swennen, R. |
| author_facet | Kisitu, J. Saikai, K.K. Coyne, D. Kanaabi, R. Kisaakye, J. Swennen, R. Nakato, G.V. |
| author_sort | Kisitu, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A high throughput in vitro screening method for phenotyping banana resistance to Radopholus similis was developed and validated using sand-MS media. The reproduction factor of R. similis showed a similar trend under in vitro and screenhouse conditions. The in vitro method proved sensitive and cost-effective for early-stage resistance screening, which favored higher nematode reproduction than in pots in the screenhouse. The in vitro method complements screenhouse screening but enables a higher throughput with a shorter turnaround time, accelerating phenotyping and enabling more rapid assessment of banana genotypes for breeding programs. Strangely, some genotypes were susceptible under in vitro but resistant under screenhouse conditions, and vice versa. Consequently, additional methods may be required, such as root necrosis scores, to validate resistance. However, this study provides the first in vitro-based demonstration of phenotyping for nematode resistance using modified sand-MS media. The modified sand-MS media appeared to provide a better environment for root development and pathogen interactions compared to gelrite and agar. By integrating both in vitro and screenhouse methods, banana breeding programs can accelerate the identification of resistant genotypes, while ensuring rigorous validation. |
| format | Journal Article |
| id | CGSpace180307 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| record_format | dspace |
| spelling | CGSpace1803072026-01-22T02:10:39Z High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis Kisitu, J. Saikai, K.K. Coyne, D. Kanaabi, R. Kisaakye, J. Swennen, R. Nakato, G.V. bananas musa nematodes screening east africa A high throughput in vitro screening method for phenotyping banana resistance to Radopholus similis was developed and validated using sand-MS media. The reproduction factor of R. similis showed a similar trend under in vitro and screenhouse conditions. The in vitro method proved sensitive and cost-effective for early-stage resistance screening, which favored higher nematode reproduction than in pots in the screenhouse. The in vitro method complements screenhouse screening but enables a higher throughput with a shorter turnaround time, accelerating phenotyping and enabling more rapid assessment of banana genotypes for breeding programs. Strangely, some genotypes were susceptible under in vitro but resistant under screenhouse conditions, and vice versa. Consequently, additional methods may be required, such as root necrosis scores, to validate resistance. However, this study provides the first in vitro-based demonstration of phenotyping for nematode resistance using modified sand-MS media. The modified sand-MS media appeared to provide a better environment for root development and pathogen interactions compared to gelrite and agar. By integrating both in vitro and screenhouse methods, banana breeding programs can accelerate the identification of resistant genotypes, while ensuring rigorous validation. 2025 2026-01-21T15:23:13Z 2026-01-21T15:23:13Z Journal Article https://hdl.handle.net/10568/180307 en Open Access application/pdf Kisitu, J., Saikai, K.K., Coyne, D., Kanaabi, R., Kisaakye, J., Swennen, R. & Nakato, G.V. (2025). High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis. Journal of Nematology, 57: e2025-1, 1-11. |
| spellingShingle | bananas musa nematodes screening east africa Kisitu, J. Saikai, K.K. Coyne, D. Kanaabi, R. Kisaakye, J. Swennen, R. Nakato, G.V. High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title | High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title_full | High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title_fullStr | High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title_full_unstemmed | High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title_short | High throughput resistance phenotyping of banana (Musa spp.) against Radopholus similis |
| title_sort | high throughput resistance phenotyping of banana musa spp against radopholus similis |
| topic | bananas musa nematodes screening east africa |
| url | https://hdl.handle.net/10568/180307 |
| work_keys_str_mv | AT kisituj highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT saikaikk highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT coyned highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT kanaabir highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT kisaakyej highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT swennenr highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis AT nakatogv highthroughputresistancephenotypingofbananamusasppagainstradopholussimilis |