Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants
The hypersensitive response (HR) is a powerful plant defence mechanism that restricts pathogen spread through localised cell death. However, constitutive activation of HR can impair growth and development, limiting its utility in crop breeding and protection. In the study, we developed a virus‐induc...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/180226 |
| _version_ | 1855527540135821312 |
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| author | Pan, Ran‐Ran Xie, Ting Wang, Ya‐Jie Song, Hong‐Da Cuellar, Wilmer Jose Xie, Yan Liu, Shu‐Sheng Wang, Xiao‐Wei |
| author_browse | Cuellar, Wilmer Jose Liu, Shu‐Sheng Pan, Ran‐Ran Song, Hong‐Da Wang, Xiao‐Wei Wang, Ya‐Jie Xie, Ting Xie, Yan |
| author_facet | Pan, Ran‐Ran Xie, Ting Wang, Ya‐Jie Song, Hong‐Da Cuellar, Wilmer Jose Xie, Yan Liu, Shu‐Sheng Wang, Xiao‐Wei |
| author_sort | Pan, Ran‐Ran |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The hypersensitive response (HR) is a powerful plant defence mechanism that restricts pathogen spread through localised cell death. However, constitutive activation of HR can impair growth and development, limiting its utility in crop breeding and protection. In the study, we developed a virus‐inducible HR system by coupling an Avirulence/Resistance ( Avr/R ) gene pair, Avr4/Cf‐4 effector‐resistance gene pair, with a promoter ( Solyc04g076730 ) specifically activated by geminivirus infection. We demonstrate that co‐expression of Avr4 and Cf‐4 in Nicotiana benthamiana triggers robust HR and significantly reduces accumulation of multiple geminiviruses, including TYLCV, TYLCCNV, SLCMV and BSCTV. Transcriptomic analysis of virus‐infected tomato identified Solyc04g076730 as a virus‐responsive promoter with minimal basal activity. Transient assays and stable transformation in both N. benthamiana and tomato revealed that this promoter effectively drives HR only upon viral infection, resulting in reduced viral loads, attenuated symptoms and improved plant vigour. Importantly, transgenic plants expressing the Solyc04g076730::Avr4/Cf‐4 construct maintained normal growth under non‐infected conditions. Our findings offer a promising strategy for engineering broad‐spectrum resistance to viral pathogens in crops. |
| format | Journal Article |
| id | CGSpace180226 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1802262026-01-21T02:08:31Z Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants Pan, Ran‐Ran Xie, Ting Wang, Ya‐Jie Song, Hong‐Da Cuellar, Wilmer Jose Xie, Yan Liu, Shu‐Sheng Wang, Xiao‐Wei plant diseases viroses hypersensitivity The hypersensitive response (HR) is a powerful plant defence mechanism that restricts pathogen spread through localised cell death. However, constitutive activation of HR can impair growth and development, limiting its utility in crop breeding and protection. In the study, we developed a virus‐inducible HR system by coupling an Avirulence/Resistance ( Avr/R ) gene pair, Avr4/Cf‐4 effector‐resistance gene pair, with a promoter ( Solyc04g076730 ) specifically activated by geminivirus infection. We demonstrate that co‐expression of Avr4 and Cf‐4 in Nicotiana benthamiana triggers robust HR and significantly reduces accumulation of multiple geminiviruses, including TYLCV, TYLCCNV, SLCMV and BSCTV. Transcriptomic analysis of virus‐infected tomato identified Solyc04g076730 as a virus‐responsive promoter with minimal basal activity. Transient assays and stable transformation in both N. benthamiana and tomato revealed that this promoter effectively drives HR only upon viral infection, resulting in reduced viral loads, attenuated symptoms and improved plant vigour. Importantly, transgenic plants expressing the Solyc04g076730::Avr4/Cf‐4 construct maintained normal growth under non‐infected conditions. Our findings offer a promising strategy for engineering broad‐spectrum resistance to viral pathogens in crops. 2025-12-01 2026-01-20T14:16:45Z 2026-01-20T14:16:45Z Journal Article https://hdl.handle.net/10568/180226 en Open Access application/pdf Wiley Pan, R.; Xie, T.; Wang, Y.; Song, H.; Cuellar, W.J.; Xie, Y.; Liu, S.; Wang, X. (2025) Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants. Molecular Plant Pathology 26(12): e70195. ISSN: 1364-3703 |
| spellingShingle | plant diseases viroses hypersensitivity Pan, Ran‐Ran Xie, Ting Wang, Ya‐Jie Song, Hong‐Da Cuellar, Wilmer Jose Xie, Yan Liu, Shu‐Sheng Wang, Xiao‐Wei Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title | Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title_full | Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title_fullStr | Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title_full_unstemmed | Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title_short | Virus‐inducible activation of hypersensitive response confers broad‐spectrum resistance to diverse viruses in plants |
| title_sort | virus inducible activation of hypersensitive response confers broad spectrum resistance to diverse viruses in plants |
| topic | plant diseases viroses hypersensitivity |
| url | https://hdl.handle.net/10568/180226 |
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