Using meristematic clumps as explants for long term conservation.
Breeding programmes but also agriculture in general depend on the availability of the widest possible genetic diversity within of a species. The conservation of genetic resources is thus extremely important Whilst for many plant species multiple conservation methods have been developed, for some spe...
| Main Authors: | , , |
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| Format: | Abstract |
| Language: | Inglés |
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2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179900 |
| _version_ | 1855518045193109504 |
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| author | Wilms, Hannes Fanega Sleziak, Natalia Panis, Bart |
| author_browse | Fanega Sleziak, Natalia Panis, Bart Wilms, Hannes |
| author_facet | Wilms, Hannes Fanega Sleziak, Natalia Panis, Bart |
| author_sort | Wilms, Hannes |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Breeding programmes but also agriculture in general depend on the availability of the widest possible genetic diversity within of a species. The conservation of genetic resources is thus extremely important Whilst for many plant species multiple conservation methods have been developed, for some species with recalcitrant seeds such as the coconut palm (Cocos nucifera) the tools are limited. However, with the recent development of a new type of explant, coconut meristematic clumps, resulting from a novel shoot micropropagation method, this type of explant could be used for the long term conservation via cryopreservation. Meristematic clumps contain multiple meristems per explants which increases the chance for survival after cryopreservation since each meristems on the explant has the potential to grow. We opted for droplet vitrification and optimized the protocol by varying preculture time and composition, PVS2 treatment time, and explant size. After cryopreservation the explants were checked if they were still able to proliferate. In the current
protocol around 50 % of explants survived liquid nitrogen exposure. However, subsequent browning during growth caused a 30% drop, resulting in 22% or less of explants that formed new meristems after cryopreservation. The material that survived was able to proliferate and regenerate into rooted plants, showing the potential of this explant for long term conservation purposes. |
| format | Abstract |
| id | CGSpace179900 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| record_format | dspace |
| spelling | CGSpace1799002026-01-16T02:03:17Z Using meristematic clumps as explants for long term conservation. Wilms, Hannes Fanega Sleziak, Natalia Panis, Bart cryobiology resource conservation Breeding programmes but also agriculture in general depend on the availability of the widest possible genetic diversity within of a species. The conservation of genetic resources is thus extremely important Whilst for many plant species multiple conservation methods have been developed, for some species with recalcitrant seeds such as the coconut palm (Cocos nucifera) the tools are limited. However, with the recent development of a new type of explant, coconut meristematic clumps, resulting from a novel shoot micropropagation method, this type of explant could be used for the long term conservation via cryopreservation. Meristematic clumps contain multiple meristems per explants which increases the chance for survival after cryopreservation since each meristems on the explant has the potential to grow. We opted for droplet vitrification and optimized the protocol by varying preculture time and composition, PVS2 treatment time, and explant size. After cryopreservation the explants were checked if they were still able to proliferate. In the current protocol around 50 % of explants survived liquid nitrogen exposure. However, subsequent browning during growth caused a 30% drop, resulting in 22% or less of explants that formed new meristems after cryopreservation. The material that survived was able to proliferate and regenerate into rooted plants, showing the potential of this explant for long term conservation purposes. 2025-05-12 2026-01-15T13:58:53Z 2026-01-15T13:58:53Z Abstract https://hdl.handle.net/10568/179900 en Open Access application/pdf Wilms, H.; Fanega Sleziak, N.; Panis, B. (2025) Using meristematic clumps as explants for long term conservation. Presentation at the 3rd Conference of Cost ACTION CA21157: Trees for the future: Cloning and beyond. Coimbra, Spain, 12–14 May, 2025, p. 32 |
| spellingShingle | cryobiology resource conservation Wilms, Hannes Fanega Sleziak, Natalia Panis, Bart Using meristematic clumps as explants for long term conservation. |
| title | Using meristematic clumps as explants for long term conservation. |
| title_full | Using meristematic clumps as explants for long term conservation. |
| title_fullStr | Using meristematic clumps as explants for long term conservation. |
| title_full_unstemmed | Using meristematic clumps as explants for long term conservation. |
| title_short | Using meristematic clumps as explants for long term conservation. |
| title_sort | using meristematic clumps as explants for long term conservation |
| topic | cryobiology resource conservation |
| url | https://hdl.handle.net/10568/179900 |
| work_keys_str_mv | AT wilmshannes usingmeristematicclumpsasexplantsforlongtermconservation AT fanegasleziaknatalia usingmeristematicclumpsasexplantsforlongtermconservation AT panisbart usingmeristematicclumpsasexplantsforlongtermconservation |