Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet
Pearl millet (Cenchrus americanus) is a drought-tolerant cereal crop. Differentially expressed genes (DEGs) were previously identified from multiple independent RNA sequencing (RNA-Seq) analyses of transcriptomes in dehydrated pearl millet plants. However, because such DEGs and their promoters were...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/179766 |
| _version_ | 1855525890505572352 |
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| author | Qazi, Maimuna Kota, Kambara Gupta, Shashi K. Takano, Tetsuo Tsugama, Daisuke |
| author_browse | Gupta, Shashi K. Kota, Kambara Qazi, Maimuna Takano, Tetsuo Tsugama, Daisuke |
| author_facet | Qazi, Maimuna Kota, Kambara Gupta, Shashi K. Takano, Tetsuo Tsugama, Daisuke |
| author_sort | Qazi, Maimuna |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pearl millet (Cenchrus americanus) is a drought-tolerant cereal crop. Differentially expressed genes (DEGs) were previously identified from multiple independent RNA sequencing (RNA-Seq) analyses of transcriptomes in dehydrated pearl millet plants. However, because such DEGs and their promoters were rarely compared between those independent analyses, upstream regulators of such transcriptomic changes remain unclear. Here, motifs in promoters of 68 sets of DEGs from publicly available 135 RNA-Seq data from unstressed and dehydrated leaves and roots were detected, which revealed that the majority of the sets of promoters of genes upregulated by such stresses contain abscisic acid (ABA) response elements. Genes potentially regulating ABA biosynthesis and signaling under such conditions were narrowed down by further analyzing the above data and newly obtained RNA-Seq data from ABA-treated plants and by reverse transcription-polymerase chain reaction. These results underscore the importance of ABA in pearl millet dehydration responses and the usefulness of promoter analyses. |
| format | Journal Article |
| id | CGSpace179766 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1797662026-01-14T02:08:07Z Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet Qazi, Maimuna Kota, Kambara Gupta, Shashi K. Takano, Tetsuo Tsugama, Daisuke abscisic acid dehydration pearl millet RNA sequencing transcription factor Pearl millet (Cenchrus americanus) is a drought-tolerant cereal crop. Differentially expressed genes (DEGs) were previously identified from multiple independent RNA sequencing (RNA-Seq) analyses of transcriptomes in dehydrated pearl millet plants. However, because such DEGs and their promoters were rarely compared between those independent analyses, upstream regulators of such transcriptomic changes remain unclear. Here, motifs in promoters of 68 sets of DEGs from publicly available 135 RNA-Seq data from unstressed and dehydrated leaves and roots were detected, which revealed that the majority of the sets of promoters of genes upregulated by such stresses contain abscisic acid (ABA) response elements. Genes potentially regulating ABA biosynthesis and signaling under such conditions were narrowed down by further analyzing the above data and newly obtained RNA-Seq data from ABA-treated plants and by reverse transcription-polymerase chain reaction. These results underscore the importance of ABA in pearl millet dehydration responses and the usefulness of promoter analyses. 2025-05-20 2026-01-13T15:27:13Z 2026-01-13T15:27:13Z Journal Article https://hdl.handle.net/10568/179766 en Open Access application/pdf Oxford University Press Qazi, Maimuna; Kota, Kambara; Gupta, Shashi K.; Takano, Tetsuo; & Tsugama, Daisuke. 2025. Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet, Bioscience, Biotechnology, and Biochemistry, Volume 89, Issue 8, August 2025, Pages 1081–1091, https://doi.org/10.1093/bbb/zbaf070 |
| spellingShingle | abscisic acid dehydration pearl millet RNA sequencing transcription factor Qazi, Maimuna Kota, Kambara Gupta, Shashi K. Takano, Tetsuo Tsugama, Daisuke Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title | Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title_full | Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title_fullStr | Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title_full_unstemmed | Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title_short | Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| title_sort | evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet |
| topic | abscisic acid dehydration pearl millet RNA sequencing transcription factor |
| url | https://hdl.handle.net/10568/179766 |
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