PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP)
This Standard Operating Procedure (SOP) describes a shotgun lipidomics method for comprehensive lipid analysis in plant and plant by-products foods implemented on a Sciex 5500 or 6500 instruments with Selexion Technology. The protocol covers extraction of 22 lipid classes from lyophilized plant mate...
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| Format: | Training Material |
| Language: | Inglés |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10568/179311 |
| _version_ | 1855538327117103104 |
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| author | Odenkirk, Melanie |
| author_browse | Odenkirk, Melanie |
| author_facet | Odenkirk, Melanie |
| author_sort | Odenkirk, Melanie |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This Standard Operating Procedure (SOP) describes a shotgun lipidomics method for comprehensive lipid analysis in plant and plant by-products foods implemented on a Sciex 5500 or 6500 instruments with Selexion Technology. The protocol covers extraction of 22 lipid classes from lyophilized plant material using a modified Bligh-Dyer method, followed by DI-DMS-MS/MS analysis with two complementary acquisition methods. The procedure includes quality control measures, DMS tuning optimization, and automated data processing through Shotgun Lipidomics Assistant software, designed specifically for the Periodic Table of Food Initiative (PTFI) to characterize lipid diversity across plant species. For unresolved issues, users are encouraged to seek additional assistance via ptfionboarding@gmail.com. |
| format | Training Material |
| id | CGSpace179311 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| record_format | dspace |
| spelling | CGSpace1793112025-12-30T02:15:33Z PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) Odenkirk, Melanie agrobiodiversity standard operating procedures lipids lipid content This Standard Operating Procedure (SOP) describes a shotgun lipidomics method for comprehensive lipid analysis in plant and plant by-products foods implemented on a Sciex 5500 or 6500 instruments with Selexion Technology. The protocol covers extraction of 22 lipid classes from lyophilized plant material using a modified Bligh-Dyer method, followed by DI-DMS-MS/MS analysis with two complementary acquisition methods. The procedure includes quality control measures, DMS tuning optimization, and automated data processing through Shotgun Lipidomics Assistant software, designed specifically for the Periodic Table of Food Initiative (PTFI) to characterize lipid diversity across plant species. For unresolved issues, users are encouraged to seek additional assistance via ptfionboarding@gmail.com. 2024-09-30 2025-12-29T08:14:54Z 2025-12-29T08:14:54Z Training Material https://hdl.handle.net/10568/179311 en Open Access application/pdf Odenkirk, M. (2024) PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP). 14 p. |
| spellingShingle | agrobiodiversity standard operating procedures lipids lipid content Odenkirk, Melanie PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title | PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title_full | PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title_fullStr | PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title_full_unstemmed | PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title_short | PTFI Lipidomics of plant food (Green Method) - Standard Operating Procedure (SOP) |
| title_sort | ptfi lipidomics of plant food green method standard operating procedure sop |
| topic | agrobiodiversity standard operating procedures lipids lipid content |
| url | https://hdl.handle.net/10568/179311 |
| work_keys_str_mv | AT odenkirkmelanie ptfilipidomicsofplantfoodgreenmethodstandardoperatingproceduresop |