Development of RAPD protocol for Shorea laevis
To study genetic diversity of Shorea leavis using RAPD, optimization of the method is necessary to obtain good resolution. Two kinds of PCR conditions, emplyoing 109 random primers of Kit B, C,E, F, G and H from Operon Technology Ltd. were tried on S. laevis DNA extracted by CTAB miniprep method. It...
| Autores principales: | , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1998
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/17929 |
| _version_ | 1855515720115290112 |
|---|---|
| author | Siregar, U.J. Sudarmonowati, E. Sri Hartati, N. |
| author_browse | Siregar, U.J. Sri Hartati, N. Sudarmonowati, E. |
| author_facet | Siregar, U.J. Sudarmonowati, E. Sri Hartati, N. |
| author_sort | Siregar, U.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | To study genetic diversity of Shorea leavis using RAPD, optimization of the method is necessary to obtain good resolution. Two kinds of PCR conditions, emplyoing 109 random primers of Kit B, C,E, F, G and H from Operon Technology Ltd. were tried on S. laevis DNA extracted by CTAB miniprep method. It was found out that the most suitable PCR condition for S. laevis is condition, which require more PCR cycles, compunded with higher MgCl2, concentration, employed more DNA sample and primer. Further survey using two primers on 110 individuals from natural habitat showed that S.laevis has considerable amount of genetic variation |
| format | Journal Article |
| id | CGSpace17929 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| record_format | dspace |
| spelling | CGSpace179292025-01-24T14:12:01Z Development of RAPD protocol for Shorea laevis Siregar, U.J. Sudarmonowati, E. Sri Hartati, N. biochemical techniques dna genetic variation polymerase chain reaction shorea laevis To study genetic diversity of Shorea leavis using RAPD, optimization of the method is necessary to obtain good resolution. Two kinds of PCR conditions, emplyoing 109 random primers of Kit B, C,E, F, G and H from Operon Technology Ltd. were tried on S. laevis DNA extracted by CTAB miniprep method. It was found out that the most suitable PCR condition for S. laevis is condition, which require more PCR cycles, compunded with higher MgCl2, concentration, employed more DNA sample and primer. Further survey using two primers on 110 individuals from natural habitat showed that S.laevis has considerable amount of genetic variation 1998 2012-06-04T09:04:45Z 2012-06-04T09:04:45Z Journal Article https://hdl.handle.net/10568/17929 en Siregar, U. J., Sudarmonowati, E., Sri Hartati, N. 1998. Development of RAPD protocol for Shorea laevis . Annales Bogorienses 5 (2) :85-92. |
| spellingShingle | biochemical techniques dna genetic variation polymerase chain reaction shorea laevis Siregar, U.J. Sudarmonowati, E. Sri Hartati, N. Development of RAPD protocol for Shorea laevis |
| title | Development of RAPD protocol for Shorea laevis |
| title_full | Development of RAPD protocol for Shorea laevis |
| title_fullStr | Development of RAPD protocol for Shorea laevis |
| title_full_unstemmed | Development of RAPD protocol for Shorea laevis |
| title_short | Development of RAPD protocol for Shorea laevis |
| title_sort | development of rapd protocol for shorea laevis |
| topic | biochemical techniques dna genetic variation polymerase chain reaction shorea laevis |
| url | https://hdl.handle.net/10568/17929 |
| work_keys_str_mv | AT siregaruj developmentofrapdprotocolforshorealaevis AT sudarmonowatie developmentofrapdprotocolforshorealaevis AT srihartatin developmentofrapdprotocolforshorealaevis |