Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat
A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they a...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/179272 |
| _version_ | 1855517762498068480 |
|---|---|
| author | Mishra, Chandra Nath Sushma Kumari, Pawar Sharma, Swati Thakur, Arun Sabhyata, Sabhyata Mishra, Shubham Kumar, Satish Gupta, Om Prakash Joshi, Arun Tiwari, Ratan |
| author_browse | Gupta, Om Prakash Joshi, Arun Kumar, Satish Mishra, Chandra Nath Mishra, Shubham Sabhyata, Sabhyata Sharma, Swati Sushma Kumari, Pawar Thakur, Arun Tiwari, Ratan |
| author_facet | Mishra, Chandra Nath Sushma Kumari, Pawar Sharma, Swati Thakur, Arun Sabhyata, Sabhyata Mishra, Shubham Kumar, Satish Gupta, Om Prakash Joshi, Arun Tiwari, Ratan |
| author_sort | Mishra, Chandra Nath |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they are expressed differently, which heavily influences BNI activity. Grain yield and 1000-grain weight were higher in BNI Munal than in Munal. All the other traits were similar in performance. Varying nitrogen dosages led to significant differences in gene expression patterns between the two genotypes. Genes related to binding and catalytic activity were prevalent among molecular functions, while genes corresponding to cellular anatomical entities dominated the cellular component category. Differential expression was observed in 371 genes at 0%N, 261 genes at 50%N, 303 genes at 75%N, and 736 genes at 100%N. Five unigenes (three upregulated and two downregulated) were consistently expressed across all nitrogen levels. Further analysis of upregulated unigenes identified links to the NrpA gene (involved in nitrogen regulation), tetratricopeptide repeat-containing protein (PPR), and cytokinin dehydrogenase 2. Analysis of downregulated genes pointed to associations with the Triticum aestivum 3BS-specific BAC library, which encodes the NPF (Nitrate and Peptide Transporter Family) and the TaVRN gene family (closely related to the TaNUE1 gene). The five unigenes and one unigene highlighted in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were validated in Munal and BNI Munal. The results obtained will enhance our understanding about gene expression patterns across different nitrogen levels in BNI wheat and help us breed wheat varieties with the BNI trait for improved NUE. |
| format | Journal Article |
| id | CGSpace179272 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1792722025-12-24T02:03:12Z Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat Mishra, Chandra Nath Sushma Kumari, Pawar Sharma, Swati Thakur, Arun Sabhyata, Sabhyata Mishra, Shubham Kumar, Satish Gupta, Om Prakash Joshi, Arun Tiwari, Ratan nitrification inhibitors transcriptomics genes wheat A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they are expressed differently, which heavily influences BNI activity. Grain yield and 1000-grain weight were higher in BNI Munal than in Munal. All the other traits were similar in performance. Varying nitrogen dosages led to significant differences in gene expression patterns between the two genotypes. Genes related to binding and catalytic activity were prevalent among molecular functions, while genes corresponding to cellular anatomical entities dominated the cellular component category. Differential expression was observed in 371 genes at 0%N, 261 genes at 50%N, 303 genes at 75%N, and 736 genes at 100%N. Five unigenes (three upregulated and two downregulated) were consistently expressed across all nitrogen levels. Further analysis of upregulated unigenes identified links to the NrpA gene (involved in nitrogen regulation), tetratricopeptide repeat-containing protein (PPR), and cytokinin dehydrogenase 2. Analysis of downregulated genes pointed to associations with the Triticum aestivum 3BS-specific BAC library, which encodes the NPF (Nitrate and Peptide Transporter Family) and the TaVRN gene family (closely related to the TaNUE1 gene). The five unigenes and one unigene highlighted in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were validated in Munal and BNI Munal. The results obtained will enhance our understanding about gene expression patterns across different nitrogen levels in BNI wheat and help us breed wheat varieties with the BNI trait for improved NUE. 2025-05-12 2025-12-23T21:14:52Z 2025-12-23T21:14:52Z Journal Article https://hdl.handle.net/10568/179272 en Open Access application/pdf MDPI Mishra, C. N., Pawar, S. K., Sharma, S., Thakur, A., Sabhyata, S., Mishra, S., Kumar, S., Gupta, O. P., Joshi, A. K., & Tiwari, R. (2025). Transcriptomic Analysis to Understand the Nitrogen Stress Response Mechanism in BNI-Enabled Wheat. International Journal of Molecular Sciences, 26(10), 4610. https://doi.org/10.3390/ijms26104610 |
| spellingShingle | nitrification inhibitors transcriptomics genes wheat Mishra, Chandra Nath Sushma Kumari, Pawar Sharma, Swati Thakur, Arun Sabhyata, Sabhyata Mishra, Shubham Kumar, Satish Gupta, Om Prakash Joshi, Arun Tiwari, Ratan Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title_full | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title_fullStr | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title_full_unstemmed | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title_short | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| title_sort | transcriptomic analysis to understand the nitrogen stress response mechanism in bni enabled wheat |
| topic | nitrification inhibitors transcriptomics genes wheat |
| url | https://hdl.handle.net/10568/179272 |
| work_keys_str_mv | AT mishrachandranath transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT sushmakumaripawar transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT sharmaswati transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT thakurarun transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT sabhyatasabhyata transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT mishrashubham transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT kumarsatish transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT guptaomprakash transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT joshiarun transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat AT tiwariratan transcriptomicanalysistounderstandthenitrogenstressresponsemechanisminbnienabledwheat |