Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize

Fusarium ear rot (FER) caused by Fusarium species severely reduces grain yield and quality of maize. Genome prediction (GP), a promising tool for quantitative trait breeding in plants and animals, uses molecular markers for capturing quantitative trait loci and predicting the genetic value of candid...

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Autores principales: Wang, Zhao, Zhang, Haoqiang, Ye, Wenchao, Han, Yuchen, Li, Huan, Zhou, Zijian, Li, Chunhui, Zhang, Xuecai, Zhang, Jianan, Chen, Jiafa, Wu, Jianyu
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/179265
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author Wang, Zhao
Zhang, Haoqiang
Ye, Wenchao
Han, Yuchen
Li, Huan
Zhou, Zijian
Li, Chunhui
Zhang, Xuecai
Zhang, Jianan
Chen, Jiafa
Wu, Jianyu
author_browse Chen, Jiafa
Han, Yuchen
Li, Chunhui
Li, Huan
Wang, Zhao
Wu, Jianyu
Ye, Wenchao
Zhang, Haoqiang
Zhang, Jianan
Zhang, Xuecai
Zhou, Zijian
author_facet Wang, Zhao
Zhang, Haoqiang
Ye, Wenchao
Han, Yuchen
Li, Huan
Zhou, Zijian
Li, Chunhui
Zhang, Xuecai
Zhang, Jianan
Chen, Jiafa
Wu, Jianyu
author_sort Wang, Zhao
collection Repository of Agricultural Research Outputs (CGSpace)
description Fusarium ear rot (FER) caused by Fusarium species severely reduces grain yield and quality of maize. Genome prediction (GP), a promising tool for quantitative trait breeding in plants and animals, uses molecular markers for capturing quantitative trait loci and predicting the genetic value of candidates for selection. In the present study, different subsets of markers and statistical methods for GP accuracy were tested in diverse inbred populations for FER resistance using a five-fold cross-validation approach. The prediction accuracy increased with an increase in the number of random markers; however, an increase in number beyond 10K did not increase the prediction accuracy. The prediction accuracy of selected markers was higher than that of random markers, and 500–1000 selected markers had the highest prediction accuracy, beyond which it slowly decreased. Although there was no difference among statistical methods when using selected markers at high prediction accuracy, significant differences were observed when using random markers. On this basis, a liquid chip named FER0.4K (liquid chip for genomic prediction of FER) containing 381 SNPs was developed for low-cost, high-throughput genotyping, with a prediction of approximately 0.82. The statistical method of genome prediction was compiled into a web-based, easy-to-use statistical analysis software using the “shiny” package in R. In summary, this study provides a foundation for FER resistance breeding in maize and offers new insights into the genetic improvement of other complex quantitative traits in plants.
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spelling CGSpace1792652025-12-24T02:02:13Z Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize Wang, Zhao Zhang, Haoqiang Ye, Wenchao Han, Yuchen Li, Huan Zhou, Zijian Li, Chunhui Zhang, Xuecai Zhang, Jianan Chen, Jiafa Wu, Jianyu marker-assisted selection maize phenotypes forecasting Fusarium ear rot (FER) caused by Fusarium species severely reduces grain yield and quality of maize. Genome prediction (GP), a promising tool for quantitative trait breeding in plants and animals, uses molecular markers for capturing quantitative trait loci and predicting the genetic value of candidates for selection. In the present study, different subsets of markers and statistical methods for GP accuracy were tested in diverse inbred populations for FER resistance using a five-fold cross-validation approach. The prediction accuracy increased with an increase in the number of random markers; however, an increase in number beyond 10K did not increase the prediction accuracy. The prediction accuracy of selected markers was higher than that of random markers, and 500–1000 selected markers had the highest prediction accuracy, beyond which it slowly decreased. Although there was no difference among statistical methods when using selected markers at high prediction accuracy, significant differences were observed when using random markers. On this basis, a liquid chip named FER0.4K (liquid chip for genomic prediction of FER) containing 381 SNPs was developed for low-cost, high-throughput genotyping, with a prediction of approximately 0.82. The statistical method of genome prediction was compiled into a web-based, easy-to-use statistical analysis software using the “shiny” package in R. In summary, this study provides a foundation for FER resistance breeding in maize and offers new insights into the genetic improvement of other complex quantitative traits in plants. 2025-06 2025-12-23T20:31:41Z 2025-12-23T20:31:41Z Journal Article https://hdl.handle.net/10568/179265 en Open Access application/pdf Elsevier Wang, Z., Zhang, H., Ye, W., Han, Y., Li, H., Zhou, Z., Li, C., Zhang, X., Zhang, J., Chen, J., & Wu, J. (2025). Development of a FER0.4 K SNP array for genomic predication of Fusarium ear rot resistance in maize. Crop Journal, 13(3), 996-1002. https://doi.org/10.1016/j.cj.2025.03.007
spellingShingle marker-assisted selection
maize
phenotypes
forecasting
Wang, Zhao
Zhang, Haoqiang
Ye, Wenchao
Han, Yuchen
Li, Huan
Zhou, Zijian
Li, Chunhui
Zhang, Xuecai
Zhang, Jianan
Chen, Jiafa
Wu, Jianyu
Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title_full Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title_fullStr Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title_full_unstemmed Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title_short Development of a FER0.4K SNP array for genomic predication of Fusarium ear rot resistance in maize
title_sort development of a fer0 4k snp array for genomic predication of fusarium ear rot resistance in maize
topic marker-assisted selection
maize
phenotypes
forecasting
url https://hdl.handle.net/10568/179265
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