Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize
Background: H2B histones play crucial roles in plant responses to biotic stress. However, to date, most research on H2B histones has focused on their roles in post-translational modification, and studies specifically investigating the intrinsic properties of these histones remain relatively limited....
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
BioMed Central Ltd.
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179261 |
| _version_ | 1855528672014893056 |
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| author | Ding, Ying Wu, Hongliang Ning, Na Yu, Haiyue Liu, Chaotian Lv, Ruoya Li, Shu Hao, Junjie Wang, Xintao Zhang, Xuecai Xie, Xin Li, Xinfeng Liu, Wende Li, Zhiqiang |
| author_browse | Ding, Ying Hao, Junjie Li, Shu Li, Xinfeng Li, Zhiqiang Liu, Chaotian Liu, Wende Lv, Ruoya Ning, Na Wang, Xintao Wu, Hongliang Xie, Xin Yu, Haiyue Zhang, Xuecai |
| author_facet | Ding, Ying Wu, Hongliang Ning, Na Yu, Haiyue Liu, Chaotian Lv, Ruoya Li, Shu Hao, Junjie Wang, Xintao Zhang, Xuecai Xie, Xin Li, Xinfeng Liu, Wende Li, Zhiqiang |
| author_sort | Ding, Ying |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: H2B histones play crucial roles in plant responses to biotic stress. However, to date, most research on H2B histones has focused on their roles in post-translational modification, and studies specifically investigating the intrinsic properties of these histones remain relatively limited. Here we identified the ZmH2B in maize (Zea mays) and investigated its role in the response of maize to infection by the Southern corn leaf blight pathogen Bipolaris maydis. Result: In this study, a nucleus-localized ZmH2B was identified from maize. To characterize the role of this histone in disease resistance, we employed virus-induced gene silencing (VIGS) and transient overexpression (VOX) to generate ZmH2B-silenced (FoMV:ZmH2B) and ZmH2B-overexpressing (FoMV:ZmH2B-VOX) lines. FoMV:ZmH2B lines showed enhanced B. maydis infection and an inhibited chitin-induced reactive oxygen species burst, whereas FoMV:ZmH2B-VOX lines exhibited the opposite effects. Furthermore, ZmH2B overexpression induced the expression of various pathogenesis-related genes, suggesting that these genes enhance resistance against B. maydis. Transcriptome analysis of ZmH2B-silenced plants revealed that the differentially expressed genes were predominantly enriched in photosynthesis-related pathways, pointing to a role for photosynthesis in B. maydis resistance. Conclusions: These results suggest that ZmH2B positively regulates maize resistance to B. maydis. |
| format | Journal Article |
| id | CGSpace179261 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central Ltd. |
| publisherStr | BioMed Central Ltd. |
| record_format | dspace |
| spelling | CGSpace1792612025-12-24T02:09:22Z Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize Ding, Ying Wu, Hongliang Ning, Na Yu, Haiyue Liu, Chaotian Lv, Ruoya Li, Shu Hao, Junjie Wang, Xintao Zhang, Xuecai Xie, Xin Li, Xinfeng Liu, Wende Li, Zhiqiang maize cochliobolus heterostrophus transcriptome biotic stress Background: H2B histones play crucial roles in plant responses to biotic stress. However, to date, most research on H2B histones has focused on their roles in post-translational modification, and studies specifically investigating the intrinsic properties of these histones remain relatively limited. Here we identified the ZmH2B in maize (Zea mays) and investigated its role in the response of maize to infection by the Southern corn leaf blight pathogen Bipolaris maydis. Result: In this study, a nucleus-localized ZmH2B was identified from maize. To characterize the role of this histone in disease resistance, we employed virus-induced gene silencing (VIGS) and transient overexpression (VOX) to generate ZmH2B-silenced (FoMV:ZmH2B) and ZmH2B-overexpressing (FoMV:ZmH2B-VOX) lines. FoMV:ZmH2B lines showed enhanced B. maydis infection and an inhibited chitin-induced reactive oxygen species burst, whereas FoMV:ZmH2B-VOX lines exhibited the opposite effects. Furthermore, ZmH2B overexpression induced the expression of various pathogenesis-related genes, suggesting that these genes enhance resistance against B. maydis. Transcriptome analysis of ZmH2B-silenced plants revealed that the differentially expressed genes were predominantly enriched in photosynthesis-related pathways, pointing to a role for photosynthesis in B. maydis resistance. Conclusions: These results suggest that ZmH2B positively regulates maize resistance to B. maydis. 2025-12 2025-12-23T19:55:25Z 2025-12-23T19:55:25Z Journal Article https://hdl.handle.net/10568/179261 en Open Access application/pdf BioMed Central Ltd. Ding, Y., Wu, H., Ning, N., Yu, H., Liu, C., Lv, R., Li, S., Hao, J., Wang, X., Zhang, X., Xie, X., Li, X., Liu, W., & Li, Z. (2025). Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize. BMC Plant Biology, 25(1) 1097. https://doi.org/10.1186/s12870-025-07020-9 |
| spellingShingle | maize cochliobolus heterostrophus transcriptome biotic stress Ding, Ying Wu, Hongliang Ning, Na Yu, Haiyue Liu, Chaotian Lv, Ruoya Li, Shu Hao, Junjie Wang, Xintao Zhang, Xuecai Xie, Xin Li, Xinfeng Liu, Wende Li, Zhiqiang Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title | Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title_full | Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title_fullStr | Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title_full_unstemmed | Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title_short | Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize |
| title_sort | histone zmh2b regulates resistance to the southern corn leaf blight pathogen bipolaris maydis in maize |
| topic | maize cochliobolus heterostrophus transcriptome biotic stress |
| url | https://hdl.handle.net/10568/179261 |
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