Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm

Background: Common wheat (Triticum aestivum L.) is one of the most widely grown and consumed cereal crops, but its complicated genome makes it difficult to study how genes affect important agronomic and yield-related traits. Genome-wide association study (GWAS) is a useful method for finding specifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Thakur, Ankita, Dhariwal, Raman, Joshi, Arun, Mishra, Vinod Kumar, Sharma, Sandeep, Singh, Mukesh Kumar, Kumar, Sundeep, Vasistha, Neeraj
Formato: Journal Article
Lenguaje:Inglés
Publicado: BioMed Central Ltd 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/179249
_version_ 1855542503521910784
author Thakur, Ankita
Dhariwal, Raman
Joshi, Arun
Mishra, Vinod Kumar
Sharma, Sandeep
Singh, Mukesh Kumar
Kumar, Sundeep
Vasistha, Neeraj
author_browse Dhariwal, Raman
Joshi, Arun
Kumar, Sundeep
Mishra, Vinod Kumar
Sharma, Sandeep
Singh, Mukesh Kumar
Thakur, Ankita
Vasistha, Neeraj
author_facet Thakur, Ankita
Dhariwal, Raman
Joshi, Arun
Mishra, Vinod Kumar
Sharma, Sandeep
Singh, Mukesh Kumar
Kumar, Sundeep
Vasistha, Neeraj
author_sort Thakur, Ankita
collection Repository of Agricultural Research Outputs (CGSpace)
description Background: Common wheat (Triticum aestivum L.) is one of the most widely grown and consumed cereal crops, but its complicated genome makes it difficult to study how genes affect important agronomic and yield-related traits. Genome-wide association study (GWAS) is a useful method for finding specific loci that control complex agronomic and yield-related traits. Results: The present investigation revealed significant phenotypic variability across the genotypes examined for all traits. The broad sense heritability (H2) for all traits ranged from 0.50 to 0.71 (Env1; 2021–2022) and 0.53 to 0.81 (Env2; 2022–2023). Using two environments’ phenotypic data, and high-throughput single-nucleotide polymorphisms (SNPs) genotypic data of 20,996 markers, we discovered 114 grain-yield-related quantitative trait loci (QTLs) and 300 associated SNP markers. Eighty-five of the identified markers were stable, consistently detected across environments (Env1 and Env2) and combined environment (CE) data, and showed a significant association with 32 different QTLs. The trait with the most associated QTLs (28) was the number of fertile tillers (NFT), with 70 markers. This was followed by 20 QTLs for each, spike length (SL) and spikelet number per spike (SPS), with 69 and 82 SNPs, respectively. Conversely, six SNPs that exhibited association with multiple traits were also identified. Twenty-nine of the total 114 identified QTLs were located in chromosomal positions where at least one marker-trait association had been previously identified. Conclusion: his study has found new SNPs, and useful QTLs that may help us to understand the biological processes behind each studied trait. Further validation in various genetic backgrounds and environments is necessary to confirm the potential utility of the significant alleles found in this study for breeding wheat varieties with improved agronomic and yield-related traits.
format Journal Article
id CGSpace179249
institution CGIAR Consortium
language Inglés
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher BioMed Central Ltd
publisherStr BioMed Central Ltd
record_format dspace
spelling CGSpace1792492025-12-24T02:17:30Z Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm Thakur, Ankita Dhariwal, Raman Joshi, Arun Mishra, Vinod Kumar Sharma, Sandeep Singh, Mukesh Kumar Kumar, Sundeep Vasistha, Neeraj agronomic characters genome-wide association studies wheat association mapping Background: Common wheat (Triticum aestivum L.) is one of the most widely grown and consumed cereal crops, but its complicated genome makes it difficult to study how genes affect important agronomic and yield-related traits. Genome-wide association study (GWAS) is a useful method for finding specific loci that control complex agronomic and yield-related traits. Results: The present investigation revealed significant phenotypic variability across the genotypes examined for all traits. The broad sense heritability (H2) for all traits ranged from 0.50 to 0.71 (Env1; 2021–2022) and 0.53 to 0.81 (Env2; 2022–2023). Using two environments’ phenotypic data, and high-throughput single-nucleotide polymorphisms (SNPs) genotypic data of 20,996 markers, we discovered 114 grain-yield-related quantitative trait loci (QTLs) and 300 associated SNP markers. Eighty-five of the identified markers were stable, consistently detected across environments (Env1 and Env2) and combined environment (CE) data, and showed a significant association with 32 different QTLs. The trait with the most associated QTLs (28) was the number of fertile tillers (NFT), with 70 markers. This was followed by 20 QTLs for each, spike length (SL) and spikelet number per spike (SPS), with 69 and 82 SNPs, respectively. Conversely, six SNPs that exhibited association with multiple traits were also identified. Twenty-nine of the total 114 identified QTLs were located in chromosomal positions where at least one marker-trait association had been previously identified. Conclusion: his study has found new SNPs, and useful QTLs that may help us to understand the biological processes behind each studied trait. Further validation in various genetic backgrounds and environments is necessary to confirm the potential utility of the significant alleles found in this study for breeding wheat varieties with improved agronomic and yield-related traits. 2025-12 2025-12-23T17:47:17Z 2025-12-23T17:47:17Z Journal Article https://hdl.handle.net/10568/179249 en Open Access application/pdf BioMed Central Ltd Thakur, A., Dhariwal, R., Joshi, A. K., Mishra, V. K., Sharma, S., Singh, M. K., Kumar, S., & Vasistha, N. K. (2025). Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm. BMC Plant Biology, 25(1), 1499. https://doi.org/10.1186/s12870-025-07263-6
spellingShingle agronomic characters
genome-wide association studies
wheat
association mapping
Thakur, Ankita
Dhariwal, Raman
Joshi, Arun
Mishra, Vinod Kumar
Sharma, Sandeep
Singh, Mukesh Kumar
Kumar, Sundeep
Vasistha, Neeraj
Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title_full Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title_fullStr Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title_full_unstemmed Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title_short Genome-wide association study for agronomic and yield-related traits in spring wheat (Triticum aestivum L.) germplasm
title_sort genome wide association study for agronomic and yield related traits in spring wheat triticum aestivum l germplasm
topic agronomic characters
genome-wide association studies
wheat
association mapping
url https://hdl.handle.net/10568/179249
work_keys_str_mv AT thakurankita genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT dhariwalraman genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT joshiarun genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT mishravinodkumar genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT sharmasandeep genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT singhmukeshkumar genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT kumarsundeep genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm
AT vasisthaneeraj genomewideassociationstudyforagronomicandyieldrelatedtraitsinspringwheattriticumaestivumlgermplasm