Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits
Finger millet (Eleusine coracana subsp. coracana) (2n = 4x = 36) remains one of the most important millets in East Africa (EA), where it was most likely domesticated along the highlands of Ethiopia and Uganda. The goal of the current study was to understand the population structure of the Ethiopian...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/179236 |
| _version_ | 1855536105546317824 |
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| author | Gebreyohannes, Adane Shimelis, Hussein Gimode, Davis M. Grandham, Prasad Valluri, Vinod Kumar Nida, Habte Moenga, Susan M. Ojiewo, Chris Ochieng Kilian, Benjamin Odeny, Damaris Achieng |
| author_browse | Gebreyohannes, Adane Gimode, Davis M. Grandham, Prasad Kilian, Benjamin Moenga, Susan M. Nida, Habte Odeny, Damaris Achieng Ojiewo, Chris Ochieng Shimelis, Hussein Valluri, Vinod Kumar |
| author_facet | Gebreyohannes, Adane Shimelis, Hussein Gimode, Davis M. Grandham, Prasad Valluri, Vinod Kumar Nida, Habte Moenga, Susan M. Ojiewo, Chris Ochieng Kilian, Benjamin Odeny, Damaris Achieng |
| author_sort | Gebreyohannes, Adane |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Finger millet (Eleusine coracana subsp. coracana) (2n = 4x = 36) remains one of the most important millets in East Africa (EA), where it was most likely domesticated along the highlands of Ethiopia and Uganda. The goal of the current study was to understand the population structure of the Ethiopian finger millet landraces and identify quantitative trait nucleotides (QTNs) and haplotypes associated with agronomic and nutritional traits. In a field evaluation across three environments, 448 genotypes were assessed for days to flowering (DTF), days to maturity (DTM), thousand seed weight (TSW), grain yield (GY), stay-green score (STG), and drought score (DrtSc). The harvested grain was analyzed for Fe and Zn contents. A subset of 391 genotypes was skim-sequenced, generating 24,112 high-quality SNPs that were employed for population structure, association mapping, and haplotype analysis. Seventy marker-trait associations were detected including 15 major QTNs with more than 30% phenotypic variance explained (PVE) for all traits except STG and GY. Pleiotropic major QTNs were identified for DTM/DTF and Fe/Zn on chromosomes 9B and 2B, respectively. Haplotype analysis of major QTNs identified 54 significant haplotype blocks and 2 additional haplotypes for a multidrug ABC transporter gene family like protein on chromosome 4A that was associated with PTH. Favorable haplotypes from pleiotropic DTM/DTF and Fe/Zn QTNs were present in 13 and 12 genotypes respectively, majority from Tigray region. Two genotypes from Tigray and one from Amhara harbored favorable haplotypes for DTM/DTF and Fe/Zn. These findings provide invaluable insights for targeted breeding to enhance finger millet resilience, nutritional profile, and yield. |
| format | Journal Article |
| id | CGSpace179236 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1792362025-12-24T02:14:15Z Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits Gebreyohannes, Adane Shimelis, Hussein Gimode, Davis M. Grandham, Prasad Valluri, Vinod Kumar Nida, Habte Moenga, Susan M. Ojiewo, Chris Ochieng Kilian, Benjamin Odeny, Damaris Achieng genetic structures finger millet genome-wide association studies nutritive value single nucleotide polymorphisms Finger millet (Eleusine coracana subsp. coracana) (2n = 4x = 36) remains one of the most important millets in East Africa (EA), where it was most likely domesticated along the highlands of Ethiopia and Uganda. The goal of the current study was to understand the population structure of the Ethiopian finger millet landraces and identify quantitative trait nucleotides (QTNs) and haplotypes associated with agronomic and nutritional traits. In a field evaluation across three environments, 448 genotypes were assessed for days to flowering (DTF), days to maturity (DTM), thousand seed weight (TSW), grain yield (GY), stay-green score (STG), and drought score (DrtSc). The harvested grain was analyzed for Fe and Zn contents. A subset of 391 genotypes was skim-sequenced, generating 24,112 high-quality SNPs that were employed for population structure, association mapping, and haplotype analysis. Seventy marker-trait associations were detected including 15 major QTNs with more than 30% phenotypic variance explained (PVE) for all traits except STG and GY. Pleiotropic major QTNs were identified for DTM/DTF and Fe/Zn on chromosomes 9B and 2B, respectively. Haplotype analysis of major QTNs identified 54 significant haplotype blocks and 2 additional haplotypes for a multidrug ABC transporter gene family like protein on chromosome 4A that was associated with PTH. Favorable haplotypes from pleiotropic DTM/DTF and Fe/Zn QTNs were present in 13 and 12 genotypes respectively, majority from Tigray region. Two genotypes from Tigray and one from Amhara harbored favorable haplotypes for DTM/DTF and Fe/Zn. These findings provide invaluable insights for targeted breeding to enhance finger millet resilience, nutritional profile, and yield. 2025-06 2025-12-23T16:27:58Z 2025-12-23T16:27:58Z Journal Article https://hdl.handle.net/10568/179236 en Open Access application/pdf Springer Gebreyohannes, A., Shimelis, H., Gimode, D. M., Grandham, P., Valluri, V. K., Nida, H., Moenga, S. M., Ojiewo, C. O., Kilian, B., & Odeny, D. A. (2025). Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits. Theoretical and Applied Genetics, 138(6), 111. https://doi.org/10.1007/s00122-025-04892-1 |
| spellingShingle | genetic structures finger millet genome-wide association studies nutritive value single nucleotide polymorphisms Gebreyohannes, Adane Shimelis, Hussein Gimode, Davis M. Grandham, Prasad Valluri, Vinod Kumar Nida, Habte Moenga, Susan M. Ojiewo, Chris Ochieng Kilian, Benjamin Odeny, Damaris Achieng Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title | Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title_full | Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title_fullStr | Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title_full_unstemmed | Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title_short | Genetic structure of Ethiopian finger millet landraces and genome-wide association mapping for agronomic and nutritional traits |
| title_sort | genetic structure of ethiopian finger millet landraces and genome wide association mapping for agronomic and nutritional traits |
| topic | genetic structures finger millet genome-wide association studies nutritive value single nucleotide polymorphisms |
| url | https://hdl.handle.net/10568/179236 |
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