An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot
Fusarium ear rot (FER), caused by Fusarium verticillioides, results in substantial yield losses and poses a significant threat to maize production worldwide. However, the genetic basis of FER resistance remains poorly understood. Utilizing QTL-seq and association analysis, we identified a gene encod...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier Inc.
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179221 |
| _version_ | 1855542759282180096 |
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| author | Dong, Chaopei Wu, Yabin Gao, Jingyang Song, Yunxia Wu, Zhaokun Wang, Zhao He, Jianju Yu, Qian Zhao, Zhonghuan Cao, Mengya Zhang, Ronghui Song, Weibin Zhang, Xuecai Jing, Pei Sun, Doudou Zhang, Huiyong Zhou, Zijian Chen, Jiafa Wu, Jianyu |
| author_browse | Cao, Mengya Chen, Jiafa Dong, Chaopei Gao, Jingyang He, Jianju Jing, Pei Song, Weibin Song, Yunxia Sun, Doudou Wang, Zhao Wu, Jianyu Wu, Yabin Wu, Zhaokun Yu, Qian Zhang, Huiyong Zhang, Ronghui Zhang, Xuecai Zhao, Zhonghuan Zhou, Zijian |
| author_facet | Dong, Chaopei Wu, Yabin Gao, Jingyang Song, Yunxia Wu, Zhaokun Wang, Zhao He, Jianju Yu, Qian Zhao, Zhonghuan Cao, Mengya Zhang, Ronghui Song, Weibin Zhang, Xuecai Jing, Pei Sun, Doudou Zhang, Huiyong Zhou, Zijian Chen, Jiafa Wu, Jianyu |
| author_sort | Dong, Chaopei |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fusarium ear rot (FER), caused by Fusarium verticillioides, results in substantial yield losses and poses a significant threat to maize production worldwide. However, the genetic basis of FER resistance remains poorly understood. Utilizing QTL-seq and association analysis, we identified a gene encoding UDP-ᴅ-glucuronate 4-epimerase 1 (ZmGAE1). A 141-base pair insertion was revealed as the natural functional variation in the promoter of ZmGAE1, which decreases its expression and enhances resistance to FER. Functional validation confirmed that ZmGAE1 acts as a negative regulator of maize resistance to FER. Notably, reduced ZmGAE1 accumulation not only improved FER resistance but also lowered fumonisin content. This effect was attributed to increased cell density within the down-placenta chalaza region, accompanied by the accumulation of galacturonic acid and pectin. Crucially, lines lacking ZmGAE1 exhibited no adverse effects on key agronomic traits and showed resistance to multiple diseases, including maize stalk rot, southern leaf blight, and seed rot. These findings highlight ZmGAE1 as a promising candidate for improving FER resistance in maize, offering a novel approach for crop protection and sustainable agriculture. |
| format | Journal Article |
| id | CGSpace179221 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier Inc. |
| publisherStr | Elsevier Inc. |
| record_format | dspace |
| spelling | CGSpace1792212025-12-22T23:23:32Z An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot Dong, Chaopei Wu, Yabin Gao, Jingyang Song, Yunxia Wu, Zhaokun Wang, Zhao He, Jianju Yu, Qian Zhao, Zhonghuan Cao, Mengya Zhang, Ronghui Song, Weibin Zhang, Xuecai Jing, Pei Sun, Doudou Zhang, Huiyong Zhou, Zijian Chen, Jiafa Wu, Jianyu fusarium maize maize stalk rot disease resistance Fusarium ear rot (FER), caused by Fusarium verticillioides, results in substantial yield losses and poses a significant threat to maize production worldwide. However, the genetic basis of FER resistance remains poorly understood. Utilizing QTL-seq and association analysis, we identified a gene encoding UDP-ᴅ-glucuronate 4-epimerase 1 (ZmGAE1). A 141-base pair insertion was revealed as the natural functional variation in the promoter of ZmGAE1, which decreases its expression and enhances resistance to FER. Functional validation confirmed that ZmGAE1 acts as a negative regulator of maize resistance to FER. Notably, reduced ZmGAE1 accumulation not only improved FER resistance but also lowered fumonisin content. This effect was attributed to increased cell density within the down-placenta chalaza region, accompanied by the accumulation of galacturonic acid and pectin. Crucially, lines lacking ZmGAE1 exhibited no adverse effects on key agronomic traits and showed resistance to multiple diseases, including maize stalk rot, southern leaf blight, and seed rot. These findings highlight ZmGAE1 as a promising candidate for improving FER resistance in maize, offering a novel approach for crop protection and sustainable agriculture. 2025-07 2025-12-22T23:23:31Z 2025-12-22T23:23:31Z Journal Article https://hdl.handle.net/10568/179221 en Open Access Elsevier Inc. Cell Press Dong, C., Wu, Y., Gao, J., Song, Y., Wu, Z., Wang, Z., He, J., Yu, Q., Zhao, Z., Cao, M., Zhang, R., Song, W., Zhang, X., Jing, P., Sun, D., Zhang, H., Zhou, Z., Chen, J., & Wu, J. (2025). An InDel insertion in the promoter of gene encoding UDP-D-glucuronate 4-epimerase 1 enhances maize resistance against Fusarium ear rot. Plant Communications, 6(7), 101380. https://doi.org/10.1016/j.xplc.2025.101380 |
| spellingShingle | fusarium maize maize stalk rot disease resistance Dong, Chaopei Wu, Yabin Gao, Jingyang Song, Yunxia Wu, Zhaokun Wang, Zhao He, Jianju Yu, Qian Zhao, Zhonghuan Cao, Mengya Zhang, Ronghui Song, Weibin Zhang, Xuecai Jing, Pei Sun, Doudou Zhang, Huiyong Zhou, Zijian Chen, Jiafa Wu, Jianyu An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title | An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title_full | An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title_fullStr | An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title_full_unstemmed | An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title_short | An InDel insertion in the promoter of a UDP-ᴅ-glucuronate 4-epimerase 1 gene enhances maize resistance to Fusarium ear rot |
| title_sort | indel insertion in the promoter of a udp d glucuronate 4 epimerase 1 gene enhances maize resistance to fusarium ear rot |
| topic | fusarium maize maize stalk rot disease resistance |
| url | https://hdl.handle.net/10568/179221 |
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