Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding
Wild relatives of wheat possess biological nitrification inhibition (BNI) capacity, which hinders soil nitrification and can be transferred to cultivated bread wheat through methods of wide crossing. The chromosome addition lines (CAL) with Lr#N translocations in chromosome 3BS of wheat were crafted...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer Nature
2025
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179217 |
| _version_ | 1855529042113986560 |
|---|---|
| author | Vishwakarma, Manish Kumar Kumar, Uttam Bhati, Pradeep Kumar Dreisigacker, Susanne Thiyagarajan, Karthikeyan Kishii, Masahiro Joshi, Arun Kumar |
| author_browse | Bhati, Pradeep Kumar Dreisigacker, Susanne Joshi, Arun Kumar Kishii, Masahiro Kumar, Uttam Thiyagarajan, Karthikeyan Vishwakarma, Manish Kumar |
| author_facet | Vishwakarma, Manish Kumar Kumar, Uttam Bhati, Pradeep Kumar Dreisigacker, Susanne Thiyagarajan, Karthikeyan Kishii, Masahiro Joshi, Arun Kumar |
| author_sort | Vishwakarma, Manish Kumar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Wild relatives of wheat possess biological nitrification inhibition (BNI) capacity, which hinders soil nitrification and can be transferred to cultivated bread wheat through methods of wide crossing. The chromosome addition lines (CAL) with Lr#N translocations in chromosome 3BS of wheat were crafted through crosses between Leymus racemosus and Triticum aestivum. Since the CAL has been shown to possess both BNI and other advantageous traits for wheat improvement, it was utilized as a donor to transfer improved BNI capacity into recently released wheat varieties as recipient parents. Marker-assisted backcrossing was deployed. We validated the Sequence Tagged Site markers developed from de-novo sequencing of the Leymus species, along with Kompetitive Allele-specific PCR markers, to identify both translocated and non-translocated lines of wheat and detect the introgressed segments in backcrossed progenies across various cross combinations. For marker validation, Genomic In-situ Hybridization was used to confirm the presence of the translocated region in the recipient lines. |
| format | Journal Article |
| id | CGSpace179217 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | CGSpace1792172025-12-22T22:25:59Z Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding Vishwakarma, Manish Kumar Kumar, Uttam Bhati, Pradeep Kumar Dreisigacker, Susanne Thiyagarajan, Karthikeyan Kishii, Masahiro Joshi, Arun Kumar wheat marker-assisted selection backcrossing leymus introgression genetic markers nitrification inhibitors Wild relatives of wheat possess biological nitrification inhibition (BNI) capacity, which hinders soil nitrification and can be transferred to cultivated bread wheat through methods of wide crossing. The chromosome addition lines (CAL) with Lr#N translocations in chromosome 3BS of wheat were crafted through crosses between Leymus racemosus and Triticum aestivum. Since the CAL has been shown to possess both BNI and other advantageous traits for wheat improvement, it was utilized as a donor to transfer improved BNI capacity into recently released wheat varieties as recipient parents. Marker-assisted backcrossing was deployed. We validated the Sequence Tagged Site markers developed from de-novo sequencing of the Leymus species, along with Kompetitive Allele-specific PCR markers, to identify both translocated and non-translocated lines of wheat and detect the introgressed segments in backcrossed progenies across various cross combinations. For marker validation, Genomic In-situ Hybridization was used to confirm the presence of the translocated region in the recipient lines. 2025-11 2025-12-22T22:25:58Z 2025-12-22T22:25:58Z Journal Article https://hdl.handle.net/10568/179217 en Limited Access Springer Nature Vishwakarma, M. K., Kumar, U., Bhati, P. K., Dreisigacker, S., Thiyagarajan, K., Kishii, M., & Joshi, A. K. (2025). Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding. Euphytica, 221(11), 188. https://doi.org/10.1007/s10681-025-03624-2 |
| spellingShingle | wheat marker-assisted selection backcrossing leymus introgression genetic markers nitrification inhibitors Vishwakarma, Manish Kumar Kumar, Uttam Bhati, Pradeep Kumar Dreisigacker, Susanne Thiyagarajan, Karthikeyan Kishii, Masahiro Joshi, Arun Kumar Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title | Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title_full | Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title_fullStr | Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title_full_unstemmed | Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title_short | Molecular markers for the identification of BNI capacity in chromosome addition lines and their use in marker-assisted breeding |
| title_sort | molecular markers for the identification of bni capacity in chromosome addition lines and their use in marker assisted breeding |
| topic | wheat marker-assisted selection backcrossing leymus introgression genetic markers nitrification inhibitors |
| url | https://hdl.handle.net/10568/179217 |
| work_keys_str_mv | AT vishwakarmamanishkumar molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT kumaruttam molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT bhatipradeepkumar molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT dreisigackersusanne molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT thiyagarajankarthikeyan molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT kishiimasahiro molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding AT joshiarunkumar molecularmarkersfortheidentificationofbnicapacityinchromosomeadditionlinesandtheiruseinmarkerassistedbreeding |