Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel

A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to f...

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Autores principales: Khalid, Maria, Khalid, Zoya, Ahmed, Zubair, Hickey, Lee, Rasheed, Awais, Gul, Alvina, Amir, Rabia
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier B.V. 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/179142
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author Khalid, Maria
Khalid, Zoya
Ahmed, Zubair
Hickey, Lee
Rasheed, Awais
Gul, Alvina
Amir, Rabia
author_browse Ahmed, Zubair
Amir, Rabia
Gul, Alvina
Hickey, Lee
Khalid, Maria
Khalid, Zoya
Rasheed, Awais
author_facet Khalid, Maria
Khalid, Zoya
Ahmed, Zubair
Hickey, Lee
Rasheed, Awais
Gul, Alvina
Amir, Rabia
author_sort Khalid, Maria
collection Repository of Agricultural Research Outputs (CGSpace)
description A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to find association between markers and traits. We characterized a collection of synthetic hexaploid wheat for root angle, seminal root numbers, and early vigor and identified loci associated with these traits using a 50 K SNP array and genotyping-by-sequencing (GBS) platforms. In total, 39 marker-trait nucleotides (QTNs) were associated with LA, RA, and RN distributed over 10 wheat chromosomes. Out of these, 11 QTNs were associated with LA, 15 QTNs were associated with RA, and 13 QTNs were associated with RN.LA showed a negative correlation with RN and a poor correlation with RA. The phenotypic values of synthetic hexaploid wheat accessions with a higher frequency of favorable alleles have been found to increase. Therefore, prioritizing the use of these synthetic wheat varieties in wheat breeding programs can be beneficial for improving root system architecture (RSA) and early vigor. These preliminary findings can be useful for pre-breeding efforts aimed at enhancing wheat adaptability to drought stress conditions.
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spelling CGSpace1791422025-12-21T22:05:32Z Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel Khalid, Maria Khalid, Zoya Ahmed, Zubair Hickey, Lee Rasheed, Awais Gul, Alvina Amir, Rabia wheat drought tolerance leaf area single nucleotide polymorphisms A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to find association between markers and traits. We characterized a collection of synthetic hexaploid wheat for root angle, seminal root numbers, and early vigor and identified loci associated with these traits using a 50 K SNP array and genotyping-by-sequencing (GBS) platforms. In total, 39 marker-trait nucleotides (QTNs) were associated with LA, RA, and RN distributed over 10 wheat chromosomes. Out of these, 11 QTNs were associated with LA, 15 QTNs were associated with RA, and 13 QTNs were associated with RN.LA showed a negative correlation with RN and a poor correlation with RA. The phenotypic values of synthetic hexaploid wheat accessions with a higher frequency of favorable alleles have been found to increase. Therefore, prioritizing the use of these synthetic wheat varieties in wheat breeding programs can be beneficial for improving root system architecture (RSA) and early vigor. These preliminary findings can be useful for pre-breeding efforts aimed at enhancing wheat adaptability to drought stress conditions. 2025-09 2025-12-21T22:05:31Z 2025-12-21T22:05:31Z Journal Article https://hdl.handle.net/10568/179142 en Limited Access Elsevier B.V. Khalid, M., Khalid, Z., Ahmed, Z., Hickey, L., Rasheed, A., Gul, A., & Amir, R. (2025). Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel. Plant Gene, 43, 100507. https://doi.org/10.1016/j.plgene.2025.100507
spellingShingle wheat
drought tolerance
leaf area
single nucleotide polymorphisms
Khalid, Maria
Khalid, Zoya
Ahmed, Zubair
Hickey, Lee
Rasheed, Awais
Gul, Alvina
Amir, Rabia
Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title_full Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title_fullStr Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title_full_unstemmed Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title_short Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel
title_sort genome wide association of root growth angle seminal root numbers and leaf area in the synthetic hexaploid wheat diversity panel
topic wheat
drought tolerance
leaf area
single nucleotide polymorphisms
url https://hdl.handle.net/10568/179142
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