Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat
Durum wheat (Triticum turgidum ssp. durum) suffers substantial yield losses from yellow rust (Puccinia striiformis) and leaf rust (Puccinia triticina). In this study, we employed genome-wide association studies (GWAS) to identify loci associated with rust resistance and used genomic selection (GS) t...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley Periodicals LLC
2025
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179138 |
| _version_ | 1855525327772581888 |
|---|---|
| author | Menor de Gaspar, Juan Domínguez Rondón, Alejandro García-Abadillo, Juan Knox, Ron Bokore, Firdissa Boyle, Kerry Ammar, Karim Huerta-Espino, Julio Berraies, Samia Meyer, Brad Zhang, Wentao Cuthbert, Richard Fobert, Pierre Ruan, Yuefeng Isidro y Sánchez, Julio |
| author_browse | Ammar, Karim Berraies, Samia Bokore, Firdissa Boyle, Kerry Cuthbert, Richard Domínguez Rondón, Alejandro Fobert, Pierre García-Abadillo, Juan Huerta-Espino, Julio Isidro y Sánchez, Julio Knox, Ron Menor de Gaspar, Juan Meyer, Brad Ruan, Yuefeng Zhang, Wentao |
| author_facet | Menor de Gaspar, Juan Domínguez Rondón, Alejandro García-Abadillo, Juan Knox, Ron Bokore, Firdissa Boyle, Kerry Ammar, Karim Huerta-Espino, Julio Berraies, Samia Meyer, Brad Zhang, Wentao Cuthbert, Richard Fobert, Pierre Ruan, Yuefeng Isidro y Sánchez, Julio |
| author_sort | Menor de Gaspar, Juan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Durum wheat (Triticum turgidum ssp. durum) suffers substantial yield losses from yellow rust (Puccinia striiformis) and leaf rust (Puccinia triticina). In this study, we employed genome-wide association studies (GWAS) to identify loci associated with rust resistance and used genomic selection (GS) to evaluate the predictive accuracy of different statistical models and phenotyping metrics (AUDPC_GDD, Angle, GDD50, and maxVar) in a Canadian durum wheat panel. The panel was evaluated in Mexico for yellow rust across three seasons near Toluca, and for leaf rust over two seasons at El Bat & aacute;n. Our GWAS identified 36 significant marker-trait associations (MTAs), including known loci (Yr30, Yr57, Yr82, YrU1, Lr16, Lr17, Lr18, and Lr65) and previously unreported regions. Yellow rust resistance was linked to loci on chromosomes 3A (602.7 Mbp) and 3B (243.4 Mbp), while leaf rust MTAs appeared on chromosomes 5A (552.8 Mbp) and 7A (570 Mbp). Candidate genes near novel MTAs encode defense-related proteins such as serine/threonine kinases and NB-ARC (nucleotide binding-Apaf-1, R proteins, and CED-4), F-box, and RIN4 (RPM1-interacting protein 4)-domain proteins. Among four scoring metrics tested, AUDPC_GDD consistently outperformed others for yellow rust, whereas maxVar was most effective for leaf rust, reflecting differences in phenotypic distribution and trait variance. Bayesian GS models (BayesB) achieved the highest prediction accuracy, but including GWAS-derived fixed effects did not improve predictions, likely due to complexities in modeling major-effect loci. These results underscore the importance of rust-specific phenotyping strategies and illustrate the difficulty of integrating GWAS into GS models to dissect complex resistance traits. |
| format | Journal Article |
| id | CGSpace179138 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley Periodicals LLC |
| publisherStr | Wiley Periodicals LLC |
| record_format | dspace |
| spelling | CGSpace1791382025-12-22T02:07:47Z Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat Menor de Gaspar, Juan Domínguez Rondón, Alejandro García-Abadillo, Juan Knox, Ron Bokore, Firdissa Boyle, Kerry Ammar, Karim Huerta-Espino, Julio Berraies, Samia Meyer, Brad Zhang, Wentao Cuthbert, Richard Fobert, Pierre Ruan, Yuefeng Isidro y Sánchez, Julio puccinia striiformis puccinia triticina genome-wide association studies genomic selection disease resistance Durum wheat (Triticum turgidum ssp. durum) suffers substantial yield losses from yellow rust (Puccinia striiformis) and leaf rust (Puccinia triticina). In this study, we employed genome-wide association studies (GWAS) to identify loci associated with rust resistance and used genomic selection (GS) to evaluate the predictive accuracy of different statistical models and phenotyping metrics (AUDPC_GDD, Angle, GDD50, and maxVar) in a Canadian durum wheat panel. The panel was evaluated in Mexico for yellow rust across three seasons near Toluca, and for leaf rust over two seasons at El Bat & aacute;n. Our GWAS identified 36 significant marker-trait associations (MTAs), including known loci (Yr30, Yr57, Yr82, YrU1, Lr16, Lr17, Lr18, and Lr65) and previously unreported regions. Yellow rust resistance was linked to loci on chromosomes 3A (602.7 Mbp) and 3B (243.4 Mbp), while leaf rust MTAs appeared on chromosomes 5A (552.8 Mbp) and 7A (570 Mbp). Candidate genes near novel MTAs encode defense-related proteins such as serine/threonine kinases and NB-ARC (nucleotide binding-Apaf-1, R proteins, and CED-4), F-box, and RIN4 (RPM1-interacting protein 4)-domain proteins. Among four scoring metrics tested, AUDPC_GDD consistently outperformed others for yellow rust, whereas maxVar was most effective for leaf rust, reflecting differences in phenotypic distribution and trait variance. Bayesian GS models (BayesB) achieved the highest prediction accuracy, but including GWAS-derived fixed effects did not improve predictions, likely due to complexities in modeling major-effect loci. These results underscore the importance of rust-specific phenotyping strategies and illustrate the difficulty of integrating GWAS into GS models to dissect complex resistance traits. 2025-12 2025-12-21T21:29:36Z 2025-12-21T21:29:36Z Journal Article https://hdl.handle.net/10568/179138 en Open Access application/pdf Wiley Periodicals LLC Menor de Gaspar, J., Domínguez Rondón, A., García‐Abadillo, J., Knox, R., Bokore, F. E., Boyle, K., Ammar, K., Huerta‐Espino, J., Berraies, S., Meyer, B., Zhang, W., Cuthbert, R. D., Fobert, P., Ruan, Y., & Isidro y Sánchez, J. (2025). Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat. The Plant Genome, 18(4), e70124. https://doi.org/10.1002/tpg2.70124 |
| spellingShingle | puccinia striiformis puccinia triticina genome-wide association studies genomic selection disease resistance Menor de Gaspar, Juan Domínguez Rondón, Alejandro García-Abadillo, Juan Knox, Ron Bokore, Firdissa Boyle, Kerry Ammar, Karim Huerta-Espino, Julio Berraies, Samia Meyer, Brad Zhang, Wentao Cuthbert, Richard Fobert, Pierre Ruan, Yuefeng Isidro y Sánchez, Julio Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title | Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title_full | Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title_fullStr | Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title_full_unstemmed | Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title_short | Mapping novel yellow and leaf rust loci and predicting resistance in cross derived Canadian durum wheat |
| title_sort | mapping novel yellow and leaf rust loci and predicting resistance in cross derived canadian durum wheat |
| topic | puccinia striiformis puccinia triticina genome-wide association studies genomic selection disease resistance |
| url | https://hdl.handle.net/10568/179138 |
| work_keys_str_mv | AT menordegasparjuan mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT dominguezrondonalejandro mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT garciaabadillojuan mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT knoxron mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT bokorefirdissa mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT boylekerry mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT ammarkarim mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT huertaespinojulio mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT berraiessamia mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT meyerbrad mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT zhangwentao mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT cuthbertrichard mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT fobertpierre mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT ruanyuefeng mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat AT isidroysanchezjulio mappingnovelyellowandleafrustlociandpredictingresistanceincrossderivedcanadiandurumwheat |