Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22
Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade. Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat. Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 we...
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier B.V.
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/179137 |
| _version_ | 1855534950217940992 |
|---|---|
| author | Chen, Tiantian Li, Lei Liu, Dan Tian, Yubing Li, Lingli Zeng, Jianq Rasheed, Awais Cao, Shuanghe Xia, Xianchun He, Zhonghu Liu, Jindong Zhang, Yong |
| author_browse | Cao, Shuanghe Chen, Tiantian He, Zhonghu Li, Lei Li, Lingli Liu, Dan Liu, Jindong Rasheed, Awais Tian, Yubing Xia, Xianchun Zeng, Jianq Zhang, Yong |
| author_facet | Chen, Tiantian Li, Lei Liu, Dan Tian, Yubing Li, Lingli Zeng, Jianq Rasheed, Awais Cao, Shuanghe Xia, Xianchun He, Zhonghu Liu, Jindong Zhang, Yong |
| author_sort | Chen, Tiantian |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade. Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat. Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in five environments. A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism (SNP) array. Six stable QTLs for black point resistance were detected, QBp.caas-2A, QBp.caas-2B1, QBp.caas-2B2, QBp.caas-2D, QBp.caas-3A, and QBp.caas-5B, which explained 2.1-28.8% of the phenotypic variances. The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22. QBp.caas-2B2, QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci, whereas QBp.caas-2A, QBp.caas-2B1 and QBp.caas-5B are likely to be new. Five kompetitive allele-specific PCR (KASP) markers, Kasp_2A_BP, Kasp_2B1_BP, Kasp_2B2_BP, Kasp_3A_BP, and Kasp_5B_BP, were validated in a natural population of 165 cultivars. The findings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding. |
| format | Journal Article |
| id | CGSpace179137 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier B.V. |
| publisherStr | Elsevier B.V. |
| record_format | dspace |
| spelling | CGSpace1791372025-12-22T02:12:41Z Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 Chen, Tiantian Li, Lei Liu, Dan Tian, Yubing Li, Lingli Zeng, Jianq Rasheed, Awais Cao, Shuanghe Xia, Xianchun He, Zhonghu Liu, Jindong Zhang, Yong candidate genes triticum aestivum pcr quantitative trait loci Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade. Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat. Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in five environments. A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism (SNP) array. Six stable QTLs for black point resistance were detected, QBp.caas-2A, QBp.caas-2B1, QBp.caas-2B2, QBp.caas-2D, QBp.caas-3A, and QBp.caas-5B, which explained 2.1-28.8% of the phenotypic variances. The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22. QBp.caas-2B2, QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci, whereas QBp.caas-2A, QBp.caas-2B1 and QBp.caas-5B are likely to be new. Five kompetitive allele-specific PCR (KASP) markers, Kasp_2A_BP, Kasp_2B1_BP, Kasp_2B2_BP, Kasp_3A_BP, and Kasp_5B_BP, were validated in a natural population of 165 cultivars. The findings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding. 2025-09 2025-12-21T21:23:55Z 2025-12-21T21:23:55Z Journal Article https://hdl.handle.net/10568/179137 en Open Access application/pdf Elsevier B.V. Chen, T., Li, L., Liu, D., Tian, Y., Li, L., Zeng, J., Rasheed, A., Cao, S., Xia, X., He, Z., Liu, J., & Zhang, Y. (2025). Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22. Journal of Integrative Agriculture, 24(9), 3311-3321. https://doi.org/10.1016/j.jia.2023.12.039 |
| spellingShingle | candidate genes triticum aestivum pcr quantitative trait loci Chen, Tiantian Li, Lei Liu, Dan Tian, Yubing Li, Lingli Zeng, Jianq Rasheed, Awais Cao, Shuanghe Xia, Xianchun He, Zhonghu Liu, Jindong Zhang, Yong Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title | Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title_full | Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title_fullStr | Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title_full_unstemmed | Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title_short | Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22 |
| title_sort | genome wide linkage mapping for black point resistance in a recombinant inbred line population of zhongmai 578 and jimai 22 |
| topic | candidate genes triticum aestivum pcr quantitative trait loci |
| url | https://hdl.handle.net/10568/179137 |
| work_keys_str_mv | AT chentiantian genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT lilei genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT liudan genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT tianyubing genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT lilingli genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT zengjianq genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT rasheedawais genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT caoshuanghe genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT xiaxianchun genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT hezhonghu genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT liujindong genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 AT zhangyong genomewidelinkagemappingforblackpointresistanceinarecombinantinbredlinepopulationofzhongmai578andjimai22 |