Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat
Background: Grain number (GN) is one of the key yield contributing factors in modern wheat (Triticum aestivum) varieties. Fruiting efficiency (FE) is a key trait for increasing GN by making more spike assimilates available to reproductive structures. Thousand grain weight (TGW) is also an important...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
BioMed Central Ltd.
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/179106 |
| _version_ | 1855535564832374784 |
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| author | Shahi, Dipendra Guo, Jia Babar, Md Ali Pradha, Sumit Avci, Muhsin McBreen, Jordan Liu, Zhao Bai, Guihua St. Amand, Paul Bernardo, Amy Reynolds, Matthew Paul Molero, Gemma Sukumaran, Sivakumar Foulkes, John Khan, Jahangir |
| author_browse | Avci, Muhsin Babar, Md Ali Bai, Guihua Bernardo, Amy Foulkes, John Guo, Jia Khan, Jahangir Liu, Zhao McBreen, Jordan Molero, Gemma Pradha, Sumit Reynolds, Matthew Paul Shahi, Dipendra St. Amand, Paul Sukumaran, Sivakumar |
| author_facet | Shahi, Dipendra Guo, Jia Babar, Md Ali Pradha, Sumit Avci, Muhsin McBreen, Jordan Liu, Zhao Bai, Guihua St. Amand, Paul Bernardo, Amy Reynolds, Matthew Paul Molero, Gemma Sukumaran, Sivakumar Foulkes, John Khan, Jahangir |
| author_sort | Shahi, Dipendra |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Grain number (GN) is one of the key yield contributing factors in modern wheat (Triticum aestivum) varieties. Fruiting efficiency (FE) is a key trait for increasing GN by making more spike assimilates available to reproductive structures. Thousand grain weight (TGW) is also an important component of grain yield. To understand the genetic architecture of FE and TGW, we performed a genome-wide association study (GWAS) in a panel of 236 US soft facultative wheats that were phenotyped in three experiments at two locations in Florida and genotyped with 20,706 single nucleotide polymorphisms (SNPs) generated from genotyping-by-sequencing (GBS). Results: FE showed significant positive associations with GN, grain yield (GY), and harvest index (HI). Likewise, TGW mostly had a positive correlation with GY and HI, but a negative correlation with GN. Eighteen marker-trait associations (MTAs) for FE and TGW were identified on 11 chromosomes, with nine MTAs within genes. Several MTAs associated with other traits were found within genes with different biological and metabolic functions including nuclear pore complex protein, F-box protein, oligopeptide transporter, and glycoside vacuolar protein. Two KASP markers showed significant mean differences for FE and TGW traits in a validation population. Conclusions: KASP marker development and validation demonstrated the utility of these markers for improving FE and TGW in breeding programs. The results suggest that optimizing intra-spike partitioning and utilizing marker-assisted selection (MAS) can enhance GY and HI. |
| format | Journal Article |
| id | CGSpace179106 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central Ltd. |
| publisherStr | BioMed Central Ltd. |
| record_format | dspace |
| spelling | CGSpace1791062025-12-20T02:13:51Z Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat Shahi, Dipendra Guo, Jia Babar, Md Ali Pradha, Sumit Avci, Muhsin McBreen, Jordan Liu, Zhao Bai, Guihua St. Amand, Paul Bernardo, Amy Reynolds, Matthew Paul Molero, Gemma Sukumaran, Sivakumar Foulkes, John Khan, Jahangir fruiting efficiency harvest index pcr genome-wide association studies wheat yields principal component analysis Background: Grain number (GN) is one of the key yield contributing factors in modern wheat (Triticum aestivum) varieties. Fruiting efficiency (FE) is a key trait for increasing GN by making more spike assimilates available to reproductive structures. Thousand grain weight (TGW) is also an important component of grain yield. To understand the genetic architecture of FE and TGW, we performed a genome-wide association study (GWAS) in a panel of 236 US soft facultative wheats that were phenotyped in three experiments at two locations in Florida and genotyped with 20,706 single nucleotide polymorphisms (SNPs) generated from genotyping-by-sequencing (GBS). Results: FE showed significant positive associations with GN, grain yield (GY), and harvest index (HI). Likewise, TGW mostly had a positive correlation with GY and HI, but a negative correlation with GN. Eighteen marker-trait associations (MTAs) for FE and TGW were identified on 11 chromosomes, with nine MTAs within genes. Several MTAs associated with other traits were found within genes with different biological and metabolic functions including nuclear pore complex protein, F-box protein, oligopeptide transporter, and glycoside vacuolar protein. Two KASP markers showed significant mean differences for FE and TGW traits in a validation population. Conclusions: KASP marker development and validation demonstrated the utility of these markers for improving FE and TGW in breeding programs. The results suggest that optimizing intra-spike partitioning and utilizing marker-assisted selection (MAS) can enhance GY and HI. 2025-01 2025-12-19T22:28:47Z 2025-12-19T22:28:47Z Journal Article https://hdl.handle.net/10568/179106 en Open Access application/pdf BioMed Central Ltd. Shahi, D., Guo, J., Babar, M. A., Pradhan, S., Avci, M., McBreen, J., Liu, Z., Bai, G., St Amand, P., Bernardo, A., Reynolds, M., Molero, G., Sukumaran, S., Foulkes, J., & Khan, J. (2025). Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat. BMC Plant Biology, 25(1), 101. https://doi.org/10.1186/s12870-025-06072-1 |
| spellingShingle | fruiting efficiency harvest index pcr genome-wide association studies wheat yields principal component analysis Shahi, Dipendra Guo, Jia Babar, Md Ali Pradha, Sumit Avci, Muhsin McBreen, Jordan Liu, Zhao Bai, Guihua St. Amand, Paul Bernardo, Amy Reynolds, Matthew Paul Molero, Gemma Sukumaran, Sivakumar Foulkes, John Khan, Jahangir Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title | Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title_full | Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title_fullStr | Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title_full_unstemmed | Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title_short | Dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index, grain number, and yield in wheat |
| title_sort | dissecting the genetic basis of fruiting efficiency for genetic enhancement of harvest index grain number and yield in wheat |
| topic | fruiting efficiency harvest index pcr genome-wide association studies wheat yields principal component analysis |
| url | https://hdl.handle.net/10568/179106 |
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